MICROWAVE FIXATION: EXAMINATION OF TEMPERATURE IN TISSUES DURING IRRADIATION (<Symposium>Microwave Fixation in Histochemistry)
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概要
- 論文の詳細を見る
The temperature during microwave irradiation in the core of tissue blocks, either 1 cm or 2 cm cubes of rat kidney and liver, was measured by plunging a sensor into the tissue blocks. The temperature of the tissue blocks was much higher than the outer media, i.e., chemical fixatives. Thus, the temperature of the outermedia did not reflect that in the tissue block to be fixed. The temperature of tissue reached 40℃ within 5〜10 sec of irradiation. The highest temperature of tissue block at 15-second irradiation, for example, was 92.5℃ (1 cm cube of kidney) and 98℃ (2 cm cube of liver), using 4 % PFA with CaCl_2 as a fixative. Microwave fixation is a practical tool for the preparation of tissue spections for either histology or cytology. However, it must be required that the irradiation is controlled so as not to produce high temperature within specimens.
- 日本組織細胞化学会の論文
著者
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Yamashita Shuji
Department Of Cardiology Gifu University Graduate School Of Medicine
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YASUDA KENJIRO
Department of Gastroenterology, Kyoto Second Red Cross Hospital
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AISO Sadakazu
Department of Cell Biology and Neuroscience, KEIO University School of Medicine
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Yamashita Shinsuke
Department Of Chemistry Naruto University Of Teacher Education
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Yamashina Shohei
Dept. Pharmaceutical Science Tohoku University
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Aiso S
Keio Univ. Tokyo Jpn
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Aiso Sadakazu
Department Of Anatomy Keio University School Of Medicine
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Yasuda K
Laboratory Of Metabolism Graduate School Of Human And Environmental Studies Kyoto University
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Yasuda Kenjiro
Department Of Anatomy Keio University
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Yamashita S
Keio Univ.
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SHIOZAWA MASAHIDE
Department of Anatomy, School of Medicine, Keio University
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Shiozawa Masahide
Department Of Anatomy School Of Medicine Keio University
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Yamashita Shuji
Department Of Anatomy Kitasato University School Of Medicine
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