AN EXPERIMENTAL STUDY ON THE APPLICATION OF MICROWAVE FIXATION TO IMMUNOHISTOCHEMICAL STUDIES (<Symposium I>Some Recent Advances in Life Sciences : Modern Histo- and Cytochemistry and Their Future)
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概要
- 論文の詳細を見る
Taking γ-glutamyl transpeptidase (γ-GT)-monoclonal anti γ-GT system as an example, the effect of microwave fixation for the immunohistochemical study was examined in rat kidney, pancreas, duodenum and liver, using a domestic microwave oven. The results were compared with that of the previous study using conventional fixatives. Best results were obtained in tissues irradiated in 4%-8% paraformaldehyde containing 10% glucose or 5%-10% polyethylene glycol 2000 at 45°-50℃. But, the immunoreaction was not demonstratable along the brush border of the duodenum. Though the microwave fixation is a useful tool for immunohistochemistry, it is necessary to adjust the condition of irradiation and the composition of fixatives to keep the physiological state of each organ or tissue well preserved and for the antigen to stay as it is originally located without loosing its antigenicity.
- 日本組織細胞化学会の論文
著者
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Yamashita Shuji
Department Of Cardiology Gifu University Graduate School Of Medicine
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YASUDA KENJIRO
Department of Gastroenterology, Kyoto Second Red Cross Hospital
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Yamashita Shinsuke
Department Of Chemistry Naruto University Of Teacher Education
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Yamashina Shohei
Dept. Pharmaceutical Science Tohoku University
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Yasuda K
Laboratory Of Metabolism Graduate School Of Human And Environmental Studies Kyoto University
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Yasuda Kenjiro
Department Of Anatomy Keio University
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Yamashita S
Keio Univ.
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SHIOZAWA MASAHIDE
Department of Anatomy, School of Medicine, Keio University
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Shiozawa Masahide
Department Of Anatomy School Of Medicine Keio University
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Yamashita Shuji
Department Of Anatomy Kitasato University School Of Medicine
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