LOCALIZATION OF SORBITOL DEHYDROGENASE IN THE MOUSE ACCESSORY REPRODUCTIVE ORGANS

概要

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It is well known that mammalian spermatozoa utilize fructose as an energy source. In the mouse, the fructose is produced mainly in the seminal vesicle and in the coagulating gland. In this paper, the precise localization of sorbitol dehydrogenase in these organs was studied using an electron microscope. The results indicate that the enzyme is evenly distributed in the cytoplasmic matrix of epithelial cells of the seminal vesicles and the coagulating glands. The light microscopic observations which were performed by early investigators indicated that the distribution of sorbitol dehydrogenase in the epithelial cells of the seminal vesicle was very uniform, while that in the coagulating gland was restricted to the apical region of the epithelial cells. This apparent difference is related not to the local difference of the cytoplasm but to the amount of the cytoplasmic matrix. Since the morphoplasm (nucleus, secretory granules, and the dilated cisternae) showed no reaction, the reaction in these regions might be relatively obscure under light microscopic observation.
日本組織細胞化学会の論文