IMMUNOHISTOCHEMICAL STUDY ON ARGINASE IN THE BOVIEN LIVER

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The distribution of arginase in the bovine liver was investigated by aid of the fluorescent antibody method. The crystalline arginase used in this study was found to be composed of five components by the Ouchterlony's gel diffusion test. This enzyme preparation was separated into five or six major fractions by the gel filtration method with Sephadex G-200. One of these fractions was attributable to bovine serum albumin, and the other two were common with the components of bovine heterogeneous organs and the liver of heterogeneous animals. Consequently, this enzyme preparation was composed of two components from the immunological point of view. The enzyme is encountered mainly in the liver cells surrounding the central vein and additionally in the cells of the indefinite area of a liver lobule. In the cytoplasm of each liver cells, the enzyme distributes itself diffusely. It is never found in the nucleus. Furthermore, the specific fluorescence is not seen also in the endothelial cells of the sinusoid, Kupffer's cells, the cells of the wall of the interlobular bile duct and in the endothelial cells of the interlobular blood vessels. The presence of arginase in the bovine kidney was not recognized in this study, though it has been already proved biochemically. Moreover, the specific fluorescence has not observed in the liver and kidney of the ureotelic animals, pork and mouse, though the extract of these organs produced several precipitin lines against anti-bovine liver arginase serum on the Ouchterlony's plate. Furthermore, the specific fluorescence has not been observed in the liver and kidney of the uricotelic animal, the chick. From these findings, it is supposed that the bovine arginase have high species specificity as well as high organ specificity.
日本組織細胞化学会の論文

IMMUNOHISTOCHEMICAL STUDY ON ARGINASE IN THE BOVIEN LIVER

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