Subcellular Distribution of Rat Liver NADPH-2,4-Dienoyl-CoA Reductase
スポンサーリンク
概要
- 論文の詳細を見る
The subcellular distribution of 2,4-dienoyl-CoA reductase (EC. 1.3.1.34,DCR) in rat liver was studied biochemically and immunochemically after the induction of clofibrate. DCR activity was mainly detected in the mitochondrial fraction by sucrose density gradient centrifugation in the livers of both normal and clofibrate-treated rats. It was also shown that the polyclonal antibody against purified DCR detected the enzyme in the mitochondrial fraction. However, the antibodies, which were affinity purified using the purified mitochondrial DCR or were epitope-selected using the fusion-polypeptide expressed from the mitochondrial cDNA clone (λgt11-RDR181), were cross-reacted with the peroxisomal DCR. These results suggest that peroxisomal DCR is immunochemically indistinguishable from mitochondrial DCR.
- 公益社団法人日本薬学会の論文
- 1996-02-15
著者
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Hirose Akihiko
Division of Risk Assessment, Biological Safety Research Center, National Institute of Health Science
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Hirose Akihiko
Division Of Risk Assessment Biological Safety Research Center National Institute Of Health Sciences
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MIZUGAKI Michinao
Department of Clinical Pharmaceutics, Tohoku Pharmaceutical University
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TOMIOKA Yoshihisa
Department of Pharmaceutical Sciences, Tohoku University Hospital and Division of Clinical Pharmacy,
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Hirose Akihiko
Division Of Toxicology National Institute Of Hygienic Sciences
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Miura Koichi
Department of Structural Analysis, National Cardiovascular Center Research Institute
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Mizugaki Michinao
Department Of Clinical Pharmaceutics Tohoku Pharmaceutical University
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Miura Koichi
Faculty Of Pharmaceutical Sciences Kanazawa University
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Miura Koichi
Department Of Structural Analysis National Cardiovascular Center Research Institute
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Edo K
Department Of Pharmaceutical Sciences Tohoku University Hospital
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Edo Kiyoto
Pharmacy Fukushima Medical University Hospital
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Tomioka Yoshihisa
Division Of Clinical Pharmacy Graduate School Of Pharmaceutical Sciences Tohoku University:laborator
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Tomioka Yoshihisa
Department Of Pharmaceutical Sciences Tohoku University Hospital
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EDO Kiyoto
Division of Pharmacy, Fukushima Medical College Hospital
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Mizugaki M
Department Of Clinical Pharmaceutics Tohoku Pharmaceutical University
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Mizugaki M
Department Of Pharmaceutical Sciences Tohoku University Hospital
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Hirose Akihiko
Division Of Risk Assessment Biological Safely Research Center National Institute Of Health Sciences
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