Absolute Quantification of Four Isoforms of the Class I Phosphoinositide-3-kinase Catalytic Subunit by Real-Time RT-PCR(Communications to the Editor)
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概要
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Class I phosphoinositide-3-kinase (PI3K) consists of four isoforms of the catalytic subunit, p110α, -β, -δ and -γ, generated from the genes PIK3CA, -B, -D and -G, respectively. These isoforms show different tissue distribution and some specific and indispensable functions in various biological pathways such as development, inflammation, autoimmunity and malignancy. In human cancers, frequent genomic amplification and gain-of-function mutations of PIK3CA were reported, which suggests an oncogenic potential. However, the role played by the other three isoforms in human cancer remains to be determined. We wanted to investigate the relationship between all the isoforms in human cancers. Here, we have established a system for the simultaneous absolute-quantification of all four isoforms by realtime reverse transcription polymerase chain reaction (RT-PCR). The reliability of this system was confirmed using three main criteria: (i) good correlation of each standard curve, (ii) high specificity of the PCR reactions and (iii) excellent reproducibility. Using this system, we investigated human monocytic leukemia cells (U937) to analyze expression of all four isoforms. The biological implications of the expression level of the four isoforms of class I PI3K catalytic subunit are discussed.
- 2007-06-01
著者
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YAMORI Takao
Division of Molecular Pharmacology, Cancer Chemotherapy Center, Japanese Foundation for Cancer Resea
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Yamori Takao
Division Of Experimental Chemotherapy Cancer Chemotherapy Center Japanese Foundation For Cancer
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Dan Shingo
Division of Molecular Pharmacology, Cancer Chemotherapy Center, Japanese Foundation for Cancer Resea
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NAKAMURA Hiroyuki
Division of Cardiology, Sakaide Municipal Hospital
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UNNO Michiaki
Division of Gastroenterological Surgery, Endocrinology and Vascular Medicine, Tohoku University Grad
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Unno Michiaki
Department Of Gastroenterological Surgery Tohoku University Graduate School Of Medicine
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Unno Michiaki
Division Of Biological Regulation And Oncology Department Of Surgery Tohoku University Graduate Scho
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Unno Michiaki
Division Of Hepato-biliary-pancreatic Surgery Tohoku University Graduate School Of Medicine
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Unno Michiaki
Division Of Gastroenterological Surgery Department Of Surgery Tohoku University School Of Medicine
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Dan Shingo
Division Of Molecular Pharmacology Cancer Chemotherapy Center Japanese Foundation For Cancer Researc
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AKASHI Tetsuyuki
Division of Molecular Pharmacology, Cancer Chemotherapy Center, Japanese Foundation for Cancer Resea
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Yamori Takao
Division Of Molecular Pharmacology Cancer Chemotherapy Center Japanese Foundation For Cancer Researc
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Akashi Tetsuyuki
Division Of Molecular Pharmacology Cancer Chemotherapy Center Japanese Foundation For Cancer Researc
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Dan Shingo
Division Of Molecular Pharmacology Cancer Chemotherapy Center Japanese Foundation For Cancer Researc
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Nakamura Hiroyuki
Division Of Molecular Pharmacology Cancer Chemotherapy Center Japanese Foundation For Cancer Researc
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Nakamura Hiroyuki
Division Of Cardiology Sakaide Municipal Hospital
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