Development and Characterization of a Model of Liver Metastasis Using Human Colon Cancer HCT-116 Cells(Highlighted paper selected by Editor-in-chief,Miscellaneous)
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概要
- 論文の詳細を見る
In order to develop a model of liver metastasis of human gastrointestinal cancer cells, we examined the potential of 10 human colon and stomach cancer cell lines (HT-29, WiDr, HCT-116, HCT-15, HCC-2998, MKN7, MKN28, MKN45, MKN74 and St-4) to form liver metastases in nude mice. Among the cell lines, HCT-116 cells consistently formed gross liver metastases when injected into the spleens of nude mice. In contrast, other human colon and stomach cancer cells produced little or no liver metastasis. In order to analyze the high metastatic potential of HCT-116 cells, the adhesion potential was compared between HCT-116 cells and the other colon cancer cell lines. HCT-116 cells showed more efficient adhesion to fibronectin (FN) than other cells. Furthermore, FN enhanced haptotaxis of HCT-116 cells, but not of other colon cancer cells. The high adhesion potential to FN and enhanced haptotaxis may contribute, at least in part, to the high metastatic potential of HCT-116. To assess the value of this newly developed model of liver metastasis, we compared the ability of four anticancer drugs (fluorouracil, doxifluridine, paclitaxel and irinotecan) to inhibit the formation of liver metastases. Paclitaxel and irinotecan showed strong inhibition of liver metastasis but fluorouracil and doxifluridine showed only slight inhibition. Therefore, this model of metastasis may be useful for screening anti-liver metastatic reagents. These results indicate that the HCT-116 liver-metastasis model should be useful for analyzing the molecular mechanism of liver metastasis and for evaluating new anti-liver metastatic drugs.
- 公益社団法人日本薬学会の論文
- 2007-09-01
著者
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YAMORI Takao
Division of Molecular Pharmacology, Cancer Chemotherapy Center, Japanese Foundation for Cancer Resea
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Yamori T
Division Of Molecular Pharmacology Cancer Chemotherapy Center Japanese Foundation For Cancer Researc
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MAKUUCHI HIROYASU
Department of Cardiovascular Surgery, Tokai University Hospital
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Yamori Takao
Division Of Experimental Chemotherapy Cancer Chemotherapy Center Japanese Foundation For Cancer
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TSURUO Takashi
Division of Experimental Chemotherapy, Cancer Chemotherapy Center, Japanese Foundation for Cancer Re
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SADAHIRO Sotaro
Department of Surgery, Tokai University School of Medicine
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Yamazaki Kanami
Division of Molecular Pharmacology, Cancer Chemotherapy Center, Japanese Foundation for Cancer Resea
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ISHIZU Kazuhiro
Division of Molecular Pharmacology, Cancer Chemotherapy Center, Japanese Foundation for Cancer Resea
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SUNOSE Naohide
Division of Experimental Chemotherapy, Cancer Chemotherapy Center, Japanese Foundation for Cancer Re
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Makuuchi Hiroyasu
Department Of Cardiovascular Surgery Tokai University Hospital
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Ishizu Kazuhiro
Division Of Molecular Pharmacology Cancer Chemotherapy Center Japanese Foundation For Cancer Researc
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Tsuruo Takashi
Division Of Experimental Chemotherapy Cancer Chemotherapy Center Japanese Foundation For Cancer Rese
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Sunose Naohide
Division Of Experimental Chemotherapy Cancer Chemotherapy Center Japanese Foundation For Cancer Rese
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Sadahiro Sotaro
Department Of Surgery Tokai University School Of Medicine
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Yamazaki Kanami
Division Of Molecular Pharmacology Cancer Chemotherapy Center Japanese Foundation For Cancer Researc
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