A Novel Differentiation Factor for PC12 Cells from Culture Supernatant of Mouse Hepatocyte Cell Line MLE-15A2

スポンサーリンク

概要

• 論文の詳細を見る

• We have found a factor that induces neurite outgrowth of rat PC12 cells in the culture supernatant of the cell line MLEL15A2. This factor was designated as MDDF. The factor was sensitive to protease, dithiothreitol, and high-temperature treatments. The apparent molecular mass was 80 kDa on Superdex 200 gel filtration. No significant tyrosine phosphorylation was detected after MDDF stimulation in Western blotting analysis with anti-phosphotyrosine antibody, suggesting that the signal transduction may not be mediated by a tyrosine kinase cascade that is involved in signaling of most of the known factors. Activation of MAP kinase was very weak and was seen only 5 min after stimulation, suggesting that prolonged activation of MAP kinase was not required for neurite outgrowth induced by MDDF. Because the biochemical characteristics of MDDF are different from those of any known peptide factors that induce neurite outgrowth of PC12 cells, MDDF may be a novel differentiation factor for PC12 cells.

• 社団法人日本農芸化学会の論文
• 1996-08-23

著者

• Kanda H

  Japanese Foundation For Cancer Res. Tokyo

• Yamori T

  Division Of Molecular Pharmacology Cancer Chemotherapy Center Japanese Foundation For Cancer Researc

• Yamori Takao

  Cancer Chemotherapy Center Japanese Foundation For Cancer Research

• Yamori Takao

  Cancer Chemotherapy Center Cancer Center

• FUKUI Yasuhisa

  Department of Applied Biological Chemistry The University of Tokyo

• Kimura Koutarou

  Department of Applied Biological Chemistry, Faculty of Agriculture and Life Science, University of T

• Kanda Hiroaki

  Cancer Institute Hospital

• FUJIYOSHI Toshihide

  Department of Applied Biological Chemistry, Graduate School of Agriculture and Life Science, The Uni

• KOMI Akiko

  Japanese Foundation for Cancer Research

• Ihara S

  Univ. Tokyo Tokyo

• IHARA Sayoko

  Department of Agriculture and Life Science, The University of Tokyo

• NISHIKAWA Tohru

  Department of Agriculture and Life Science, The University of Tokyo

• SHIRAI Toshiyuki

  Department of Agriculture and Life Science, The University of Tokyo

• KOMI Akiko

  Cancer Chemotherapy Center, Japanese Foundation for Cancer Research

• Ihara Seiji

  Department Of Molecular Life Science 2 School Of Medicine Tokai University

• Fukui Y

  Research Center For Environmental Genomics Kobe University

• Kanda Hiroaki

  Department Of Pathology Cancer Institute Hospital

• Nishikawa Tohru

  Department Of Agriculture And Life Science The University Of Tokyo

• Fukui Yuko

  Institute For Fundamental Research Suntory Ltd. (sunbor)

• Kimura Koutarou

  Department Of Agriculture And Life Science The University Of Tokyo

• Shirai Toshiyuki

  Department Of Agriculture And Life Science The University Of Tokyo

• Fujiyoshi T

  Univ. Tokyo Tokyo

• Fujiyoshi Toshihide

  Department Of Applied Biological Chemistry Graduate School Of Agriculture And Life Science The Unive

• Yamori Takao

  Cancer Chemotherapy Center

関連論文

• Morphology of Ras- Transformed Cells Becomes Apparently Normal Again with Tyrosine Kinase Inhibitors without a Decrease in the Ras- GTP Complex^1
• Phosphatidylinositol-3 Kinase in Fission Yeast : A Possible Role in Stress Responses
• Analysis of extracellular matrix of a skin tumor from patients with juvenile hyaline fibromatosis
• Marine Diterpenoids with a Briarane Skeleton from the Okinawan Soft Coral Pachyclavularia violacea
• Activation of Protein Kinase B Induced by H_2 O_2 and Heat Shock through Distinct Mechanisms Dependent and Independent of Phosphatidylinositol 3-Kinase
• Identification of Protein Kinase B (PKB) as a Phosphatidylinositol 3,4,5-Trisphosphate Binding Protein in Dictyostelium discoideum
• Isolation of Peridinin-Related Norcarotenoids with Cell Growth-Inhibitory Activity from the Cultured Dinoflagellate of Symbiodinium sp., a Symbiont of the Okinawan Soft Coral Clavularia viridis, and Analysis of Fatty Acids of the Dinoflagellate
• New Biologically Active Marine Sesquiterpenoid and Steroid from the Okinawan Sponge of the Genus Axinyssa
• Isolation, Characterization, and Sugar Chain Structure of EndoPG Ia, Ib, and Ic from Stereum purpureum
• Novel Exopolygalacturonases Produced by Alternaria mali
• Deoxynybomycin Is a Selective Anti-Tumor Agent Inducing Apoptosis and Inhibiting Topoisomerase I
• Identification of Interleukin-6 as a Factor That Induces Neurite Outgrowth by PC12 Cells Primed with NGF^1
• Association of Loss of Heterozygosity at the p53 Locus with Chemoresistance in Osteosarcomas
• Expression of Carbohydrate-Binding Protein p33/41 in Human Tumor Cell Lines
• Expression of Sulfomucins in Normal Mucosae, Colorectal Adenocarcinomas, and Metastases
• A Novel Differentiation Factor for PC12 Cells from Culture Supernatant of Mouse Hepatocyte Cell Line MLE-15A2
• Establishment of a Hepatocyte Cell Line Producing Growth-promoting Factors for Liver-colonizing Tumor Cells
• New Bisindole Alkaloids Isolated from Myxomycetes Arcyria cinerea and Lycogala epidendrum
• Contrast-enhanced sonography of pancreatic ductal carcinoma using agent detection imaging
• Structural Revision of Sulfated Polysaccharide B-1 Isolated from a Marine Pseudomonas Species and Its Cytotoxic Activity Against Human Cancer Cell Lines
• Development and Characterization of a Model of Liver Metastasis Using Human Colon Cancer HCT-116 Cells(Highlighted paper selected by Editor-in-chief,Miscellaneous)
• The polar neutral and basic taxoids isolated from needles and twigs of Taxus cuspidata and their biological activity
• Cloning and Expression of Human Anti-Tumor Necrosis Factor-α Monoclonal Antibodies from Epstein-Barr Virus Transformed Oligoclonal Libraries
• Epidermal Growth Factor Receptor Phosphorylates Protein Kinase C δ at Tyr332 to form a Trimeric Complex with p66Shc in the H_2O_2-stimulated Cells
• Isolation of Suppressor Mutants of Phosphatidylinositol 3-Phosphate 5-kinase Deficient Cells in Schizosaccharomyces pombe(Microbiology & Fermentation Technology)
• Inportance of Phosphatidylinositol 3-Phosphate in Sporulation of Schizosaccharomyces pombe(Biochemistry & Molecular Biology)
• Use of a Glycoprotein gB Promoter for Expression of Genes Inserted into the Human Cytomegalovirus Genome
• Leptosins isolated from marine fungus Leptoshaeria species inhibit DNA topoisomerases I and/or II and induce apoptosis by inactivation of Akt/protein kinase B
• New Dolabellane-Type Diterpenoids from the Okinawan Soft Coral of the Genus Clavularia
• Identification of the Major Antioxidative Metabolites in Biological Fluids of the Rat with Ingested (+)-Catechin and (-)-Epicatechin
• Suppression of Postprandial Hypertriglyceridemia in Rats and Mice by Oolong Tea Polymerized Polyphenols
• Up-reguration of p27^ Correlates Inversely with Anchorage-independent Growth of Human Cancer Cell Lines
• Histochemical Detection of Sugar Residues by Joint Use of Specific Antibodies and Lectins

もっと見る 閉じる

スポンサーリンク