Structural Analysis of N-Linked Carbohydrate Chains of Funnel Web Spider (Agelenopsis aperta) Venom Peptide Isomerase
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概要
- 論文の詳細を見る
The structure of the N-linked carbohydrate chains of peptide isomerase from the venom of the funnel web spider (Agelenopsis aperta) has been analyzed. Carbohydrates were released from peptide isomerase by hydrazinolysis and reductively aminated with 2-aminopyridine. The fluorescent derivatives were purified by phenol/chloroform extraction, followed by size-exclusion HPLC. The structure of the purified pyridylamino (PA-) carbohydrate chains were analyzed by a combination of two-dimensional HPLC mapping, sugar composition analysis, sequential ex-oglycosidase digestions, and mass spectrometry. The peptide isomerase contains six kinds of N-linked carbohydrate chains of truncated high-mannose type, with a fucose α1-6 linked to the reducing N-acetylglucosamine in approximately 80% of them.
- 社団法人日本農芸化学会の論文
- 1998-06-23
著者
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Mano Nariyasu
Dep. Of Pharmaceutical Sciences Tohoku Univ. Hospital
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Mano Nariyasu
東北大学 薬研究
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Mano N
Tsukuba Research Laboratories Eisai Co. Ltd.
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Asakawa N
Eisai Co. Ltd. Ibaraki Jpn
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Mano N
Dep. Of Pharmaceutical Sciences Tohoku Univ. Hospital
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Asakawa N
Eisai Tsukuba Research Laboratories
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Asakawa Naoki
Analytical Chemistry And Pharmaceutical Research Division Tsukuba Research Laboratories Eisai Co. Lt
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Asakawa Naoki
Tsukuba Research Laboratories Eisai Co. Ltd.
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Asakawa Naoki
Department Of Biomolecular Engineering Tokyo Institute Of Technology
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Asakawa Naoki
Preclinical Research Laboratories Eisai Co. Ltd.
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SHIKATA Yasushi
Tsukuba Research Laboratories, Eisai Co. Ltd.
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OHE Hiroshi
Tsukuba Research Laboratories, Eisai Co. Ltd.
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MANO Nariyasu
Tsukuba Research Laboratories, Eisai Co. Ltd.
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KUWADA Manabu
Tsukuba Research Laboratories, Eisai Co. Ltd.
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Mano Nariyasu
Analytical Chemistry And Pharmaceutical Research Section Tsukuba Research Laboratories Eisai Co. Ltd
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Ohe H
Tsukuba Research Laboratories Eisai Co. Ltd.
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Kuwada M
Tsukuba Research Laboratories Eisai Co. Ltd.
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Kuwada Manabu
Tsukuba Research Laboratories Eisai Co. Ltd.
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Shikata Y
Tsukuba Research Laboratories Eisai Co. Ltd.
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