Synthetic Cationic Amphiphile for Liposome-Mediated DNA Transfection with Less Cytotoxicity

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概要

• 論文の詳細を見る

• A cationic peptide amphiphile comprising an L-alanine residue interposed between a charged head group and a double-chain segment, N, N-dihexadecyl-Nα-[6-(trimethylammonio)-hexanoyl]-L-alaninamide bromide (NC_5-Ala2C_<16>), was synthesized and used to prepare sonicated liposomes. We examined the efficiency of this liposome in gene transfer according to the transient expression of chloramphenicol acetyltransferase (CAT). This cationic liposome reagent facilitates efficient DNA transfection in COS-7 cells. We determined the optimum conditions for NC_5Ala2C_<16> liposome-mediated transfection. The optimal amounts of the amphiphile and plasmid DNA were determined to be about 100 μg and 10 μg per 35-mm dish, respectively. The activity of this liposome was greater than that of commercial reagents, lipofectin, and N-[1-(2,3-dioleoyloxy)propyl]-N, N, N-trimethyl-ammonium methylsulfate (DOTAP), and it was less toxic than lipofectin and DOTAP in COS-7 cells.

• 社団法人日本薬学会の論文
• 1996-06-15

著者

• TANAKA Isao

  Department of materials science and engineering, Kyoto University

• MURAKAMI Yukito

  Department of Chemical Science and Technology, Faculty of Engineering, Kyushu University

• Murakami Yukito

  Department Of Chemical Science And Technology Faculty Of Engineering Kyushu University

• Murakami Yukito

  Department Of Organic Synthesis Faculty Of Engineering Kyushu University

• KATO Takashi

  Department of Pharmaceutical Research, Tsukuba Research Laboratories, Eisai Co., Ltd.,

• IWAMOTO Kiyoshi

  Department of Pharmaceutical Research, Tsukuba Research Laboratories, Eisai Co., Ltd.,

• ANDO Hidenobu

  Department of Pharmaceutical Research, Tsukuba Research Laboratories, Eisai Co., Ltd.,

• ASAKAWA Naoki

  Department of Pharmaceutical Research, Tsukuba Research Laboratories, Eisai Co., Ltd.,

• KIKUCHI Junichi

  Department of Organic Synthesis, Faculty of Engineering, Kyushu University

• Ando H

  Analytical Chemistry And Pharmaceutical Research Division Tsukuba Research Laboratories Eisai Co. Lt

• Ando Hidenobu

  エーザイ筑波研究所

• Iwamoto Kiyoshi

  Department Of Pharmaceutical Research Tsukuba Research Laboratories Eisai Co. Ltd.

• Asakawa N

  Eisai Co. Ltd. Ibaraki Jpn

• Tanaka Isao

  Department Of Materials Science And Engineering Kyoto University

• Asakawa N

  Eisai Tsukuba Research Laboratories

• Asakawa Naoki

  Analytical Chemistry And Pharmaceutical Research Division Tsukuba Research Laboratories Eisai Co. Lt

• Asakawa Naoki

  Department Of Biomolecular Engineering Tokyo Institute Of Technology

• Asakawa Naoyuki

  Institute For Chemical Research Kyoto University

• Kato Takashi

  Department Of Brain Science And Molecular Imaging National Institute For Longevity Sciences

• Asakawa Naoki

  Preclinical Research Laboratories Eisai Co. Ltd.

• Kikuchi Junichi

  Department Of Organic Synthesis Faculty Of Engineering Kyushu University

• Kato T

  Trans‐century Pharmaceutical Co. Ltd. Fuji Jpn

• Tanaka Isao

  Department Of Internal Medicine Suzuka Kaisei Hospital

• Tanaka Isao

  Department Of Pharmaceutical Research Tsukuba Research Laboratories Eisai Co. Ltd.

• Kato Takashi

  Department Of Applied Chemistry Graduate School Of Engineering Nagoya Institute Of Technology

• Kikuchi Junichi

  Department of Hydrocarbon Chemistry, Faculty of Engineering, Kyoto University

• Iwamoto Kiyoshi

  Department of Industrial Chemistry, Faculty of Engineering, Nagasaki University

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