Fusogenic behavior of single-walled vesicles composed of synthetic peptide lipids.
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概要
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Fusogenic behavior of the single-walled vesicles formed with mixed or individual synthetic peptide lipids, N<SUP>+</SUP>C<SUB>5</SUB>Ala2C<I><SUB>n</SUB></I> and (SO<SUB>3</SUB><SUP>−</SUP>)C<SUB>5</SUB>Ala2C<I><SUB>n</SUB></I> (<I>n</I>=14,16), was investigated in the presence of various water-soluble polymers or a nonbilayer-forming lipid by means of electron microscopy, differential scanning calorimetry, and turbidity measurements. The following ionic polymers having opposite charges to the net surface charges of the vesicles immediately induced fusion via vesicle aggregation; heparin, chondroitin sulfate C, chondroitin, and potassium salt of poly(vinyl sulfate) for the cationic vesicles, and polybrene for the anionic ones. On the other hand, ionic polymers having electrostatic charges identical with the vesicular surface charges and nonionic ones, such as amylose, poly(vinyl alcohol), polyethylene glycol 20000, and polyethylene glycol 6000, induced neither fusion nor aggregation. A nonbilayer-forming lipid, QC<SUB>5</SUB>Ala2C<SUB>14</SUB>, incorporated into the N<SUP>+</SUP>C<SUB>5</SUB>Ala2C<I><SUB>n</SUB></I> vesicles induced fusion at kinetic rates much slower than those triggered by the ionic polymers. The observations were discussed with attention to the dehydration effect and the participation of nonbilayer structures in the fusion process.
- 公益社団法人 日本化学会の論文
著者
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Murakami Yukito
Department Of Chemical Science And Technology Faculty Of Engineering Kyushu University
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KIKUCHI Jun-ichi
Department of Applied Chemistry, Faculty of Science and Engineering, Saga University
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Takaki Toshihiko
Department of Organic Synthesis, Faculty of Engineering, Kyushu University
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