Identification of a High-Copy-Number Plasmid Suppressor of a Lethal Phenotype Caused by Mutant DnaA Protein Which Has Decreased Instrinsic ATPase Activity
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概要
- 論文の詳細を見る
The induction of a mutant DnaA protein (DnaA E204Q) with decreased intrinsic ATPase activity in cells causes a lethal phenotype. Based on our results that the decreased ATPase activity of DnaA E204Q is activated to the level of a wild-type protein in the presence of partially purified stimulating factors for DnaA ATPase, we tested the hypothesis that genes encoding the stimulating factors can be cloned as high-copy-number plasmid suppressors for the lethality caused by DnaA E204Q. We isolated a number of high copy-number plasmids that suppress the lethal phenotype. The genes responsible for the suppression of the lethal phenotype were revealed to be dnaN, relA, pcnB and cyaA by subcloning, and a site-directed mutational analysis. The mechanisms by which these genes suppressed the lethal phenotype were examined in vivo and in vitro.
- 公益社団法人日本薬学会の論文
- 1999-09-15
著者
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土屋 照雄
Gifu Prefectural Hospital
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Tsuchiya Tomofusa
岡山大学 薬学部
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Tsuchiya Tomofusa
Faculty Of Pharmaceutical Sciences Okayama University
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Mizushima Tohru
Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University
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Miki T
Faculty Of Pharmaceutical Sciences Kyushu University
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Tsuchiya T
Faculty Of Pharmaceutical Sciences Okayama University
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Tsuchiya T
Okayama Univ. Okayama
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MIZUSHIMA Tohru
Faculty of Pharmaceutical Sciences, Okayama University
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Sakamoto Kenji
Faculty Of Pharmaceutical Sciences Okayama University
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Makise Masaki
Faculty of Pharmaceutical Sciences, Okayama University
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Makise Masaki
Graduate School Of Medical And Pharmaceutical Sciences Kumamoto University
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Tsuchiya T
Faculty Of Pharmaceutical Sciences Hokuriku University
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Mizushima Tohru
Department Of Molecular Microbiology Graduate School Of Medicine Dentistry And Pharmaceutical Scienc
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Mizushima Tohru
Faculty Of Pharmaceutial Sciences Kyushu University
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Mizushima Tohru
Faculty Of Engineering. Tokai University
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Tsuchiya Tomofusa
Department Of Microbiology Fujita Health University
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Sakamoto Kenji
Institute Of Fluid Science Tohoku University
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Mizushima T
Faculty Of Pharmaceutial Sciences Kyushu University
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Makise Masaki
Faculti of Pharmaceutical Sciences Okairama University
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Mizushima Tohru
Faculti of Pharmaceutical Sciences Okairama University
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