Sequence-Independent DNA Binding Activity of DnaA Protein, the Initiator of Chromosomal DNA Replication in Escherichia coli.
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概要
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The DnaA protein specifically binds to the origin of chromosomal DNA replication and initiates DNA synthesis. In addition to this sequence-specific DNA binding, DnaA protein binds to DNA in a sequence-independent manner. We here compared the two DNA binding activities. Binding of ATP and ADP to DnaA inhibited the sequence-independent DNA binding, but not sequence-specific binding. Sequence-independent DNA binding, but not sequence-specific binding, required incubation at high temperatures. Mutations in the C-terminal domain affected the sequence-independent DNA binding activity less drastically than they did the sequence-specific binding. On the other hand, the mutant DnaA 433, which has mutations in a membrane-binding domain (K 327 to I 344) was inert for sequence-independent binding, but could bind specifically to DNA. These results suggest that the two DNA binding activities involve different domains and perform different functions from each other in Escherichia coli cells.
- 社団法人 日本生化学会の論文
著者
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Tsuchiya Tomofusa
Faculty Of Pharmaceutical Sciences Okayama University
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Makise Masaki
Faculti of Pharmaceutical Sciences Okairama University
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Mizushima Tohru
Faculti of Pharmaceutical Sciences Okairama University
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