Preparation and Characterization of Everted Membrane Vesicles from Cells of Staphylococcus aureus
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概要
- 論文の詳細を見る
We developed a method for the preparation of everted membrane vesicles from cells of Staphylococcus aureus. The cells were first treated with ampicillin to weaken the peptidoglycan layer, then the cells were passed through a French press cell. The resulting vesicles were roughly 0.1μm in diameter, judging from electron microscopic observations. We detected fairly high membrane-bound ATPase activity in the membrane vesicles. We observed respiratory-driven quenching of quinacrine fluorescence, which indicates that inward H^+ transport took place. These results indicate that the vesicles are everted. We characterized the membrane-bound ATPase. We also detected Na^+/H^+ antiport, erythromycin/H^+ antiport and chloramphenicol/H^+ antiport activities in the membranes of S. aureus.
- 社団法人日本薬学会の論文
- 1998-01-15
著者
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TSUCHIYA Tomofusa
Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Scie
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Tsuchiya Tomofusa
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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KODAMA Kazuyo
Department of Microbiology Faculty of Pharmaceutical Sciences, Okayama University
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HASHIMOTO Atsuko
Department of Microbiology Faculty of Pharmaceutical Sciences, Okayama University
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MORITA Yuji
Department of Microbiology Faculty of Pharmaceutical Sciences, Okayama University
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TOMOCHIKA Ken-ichi
Department of Environmental Hygiene, Faculty of Pharmaceutical Sciences, Okayama University
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Morita Yuji
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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Kodama Kazuyo
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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Hashimoto Atsuko
Department Of Microbiology Faculty Of Pharmaceutical Sciences Okayama University
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Tomochika Ken-ichi
Department Of Environmental Hygiene Faculty Of Pharmaceutical Sciences Okayama University
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Morita Yuji
Department Of Biology Faculty Of Science Kumamoto University
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Morita Yuji
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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TOMOCHIKA KEN-ICHI
Department of Biology, Faculty of Science, Okayama University
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TSUCHIYA Tomofusa
Department of Microbiology Faculty of Pharmaceutical Sciences, Okayama University
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