The Protein Encoded by NCgl1221 in Corynebacterium glutamicum Functions as a Mechanosensitive Channel
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概要
- 論文の詳細を見る
The function of the NCgl1221-encoded protein of Corynebacterium glutamicum was analyzed using Bacillus subtilis as host because a method for preparing the giant provacuole required for electrophysiological studies has been established. Expression of NCgl1221 in a strain deficient in mscL and ykuT, both of which encode mechanosensitive channels, resulted in an 8.9-fold higher cell survival rate upon osmotic downshock than the control. Electrophysiological investigation showed that the giant provacuole prepared from this strain, expressing NCgl1221, exhibited significantly higher pressure-dependent conductance than the control. These findings show that the NCgl1221-encoded protein functions as a mechanosensitive channel.
- 2010-12-23
著者
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HASHIMOTO Ken-ichi
Department of Environmental Materials Science, Tokyo Denki University
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Nakamura Koji
Department Of Community Preventive Medicine Niigata University Graduate School Of Medical And Dental
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Kuroda Teruo
Department Of Microbiology Faculty Of Pharmaceutical Science Okayama University
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Kawasaki Hisashi
Department Of Green And Sustainable Chemistry Tokyo Denki University
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Kawasaki Hisashi
Department Of Environmental Materials Science Tokyo Denki University
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Nakamatsu Tsuyoshi
Department Of Green And Sustainable Chemistry Tokyo Denki University
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Nakamatsu Tsuyoshi
Department Of Environmental Materials Science Tokyo Denki University
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Hashimoto Ken-ichi
Department Of Green And Sustainable Chemistry Tokyo Denki University
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YABE Isamu
Department of Green and Sustainable Chemistry, Tokyo Denki University
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Yabe Isamu
Department Of Green And Sustainable Chemistry Tokyo Denki University
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Nakamura Koji
Department Of Microbiology Faculty Of Pharmaceutical Science Okayama University
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Hashimoto Ken-ichi
Department Of Civil Engineering And Architecture The Tokuyama College Of Technology
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Hashimoto Ken-ichi
Department of Civil Engineering and Architecture
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Nakamatsu Tsuyoshi
Department of Agricultural Chemistry, The University of Tokyo
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Kuroda Teruo
Department of Genome Applied Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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