Microbial production of 3a.ALPHA.-H-4.ALPHA.-(3'-propionic acid)-5.ALPHA.-hydroxy-7a.BETA.-methylhexahydro-1-indanone-.DELTA.-lactone from soybean sterol.

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概要

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• Fermentative production of 3aα-H-4α-(3'-propionic acid)-5a-hydroxy-7αβ-methylhexahydro-1-indanone-d-lactone (HIL) from soybean sterol was studied in order to use it as an intermediate for chemical synthesis of 19-norsteroids. A mutant of Nocardia corallina converted 20 g/liter of soybean sterol into 2.8 g/liter of HIL with a 25% yield on a molar basis.The dominant factors improving the productivity were the use of an amino acid mixture as a nitrogen source and the preparation of the sterol suspension by sonication or with surfaceactive agents.

• 公益社団法人 日本農芸化学会の論文

著者

• Nakamatsu Tsuyoshi

  Department of Agricultural Chemistry, The University of Tokyo

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• Production of (R)-3-Amino-3-phenylpropionic Acid and (S)-3-Amino-3-phenylpropionic Acid from (R,S)-N-Acetyl-3-amino-3-phenylpropionic Acid Using Microorganisms Having Enantiomer-Specific Amidohydrolyzing Activity
• Glutamate Is Excreted Across the Cytoplasmic Membrane through the NCgl1221 Channel of Corynebacterium glutamicum by Passive Diffusion
• Microbial production of 3a.ALPHA.-H-4.ALPHA.-(3'-propionic acid)-5.ALPHA.-hydroxy-7a.BETA.-methylhexahydro-1-indanone-.DELTA.-lactone from soybean sterol.

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