Glutamate Is Excreted Across the Cytoplasmic Membrane through the NCgl1221 Channel of Corynebacterium glutamicum by Passive Diffusion
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概要
- 論文の詳細を見る
The NCgl1221 gene, which encodes a mechanosensitive channel, has been reported to be critically involved in glutamate (Glu) overproduction by Corynebacterium glutamicum, but direct evidence of Glu excretion through this channel has not yet been provided. In this study, by electrophysiological methods, we found direct evidence of Glu excretion through this channel by passive diffusion. We found that the introduction into Phe-producing Escherichia coli of mutant NCgl1221 genes that induce Glu overproduction by C. glutamicum improved productivity. This suggests a low-substrate preference of this channel, indicates its potential as a versatile exporter, and more broadly, indicates the potential of exporter engineering.
著者
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Murata Jun
Department Of Applied Physics Faculty Of Engineering University Of Tokyo:presently At Musashi Works
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Kawasaki Hisashi
Department Of Environmental Materials Science Tokyo Denki University
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Nakamatsu Tsuyoshi
Department Of Environmental Materials Science Tokyo Denki University
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YABE Isamu
Department of Green and Sustainable Chemistry, Tokyo Denki University
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Yabe Isamu
Department Of Green And Sustainable Chemistry Tokyo Denki University
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Hashimoto Ken-ichi
Department Of Civil Engineering And Architecture The Tokuyama College Of Technology
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Konishi Tomoyuki
Department Of Green And Sustainable Chemistry Tokyo Denki University
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HASHIMOTO Ken-ichi
Department of Green and Sustainable Chemistry, Tokyo Denki University
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Nakamatsu Tsuyoshi
Department of Green and Sustainable Chemistry, Tokyo Denki University
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Kawasaki Hisashi
Department of Green and Sustainable Chemistry, Tokyo Denki University
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Hashimoto Ken-ichi
Department of Civil Engineering and Architecture
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