Effect of Amplification of Desensitized purF and prs on Inosine Accumulation in Escherichia coli(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

概要

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The effect of a phosphoribosylpyrophosphate(PRPP) synthetase gene(prs) that was desensitized to feedback inhibition by ADP on inosine accumulation was investigated using an inosineproducing mutant of Escherichia coli. At the same time, various types of plasmid having a PRPP amidotransferase gene(purF) that was desensitized to feedback inhibition by AMP and GMP were also investigated to improve inosine productivity using a compatible plasmid containing prs with a plasmid containing purF. The recombinant E. coli 1-9 harboring a low-copy-number plasmid having the desensitized-pwrF(pMWKQ) accumulated 3.6 g/l inosine from 40 g/l glucose in a 2-d culture. Furthermore, desensitized-prs amplification, in addition to purF, resulted in the accumulation of 6.2 g/l inosine. Additionally, through these experiments, a spontaneous mutant with an enhanced inosine-producing ability compared with the parent strain I-9 was obtained. The spontaneous mutant I-9m harboring only pMWKQ and I-9m harboring both pMWKQ and pSTVDA(a plasmid having the desensitized-prs) accumulated 6.7 g/l and 7.5 g/l inosine, respectively, from 40 g/l glucose in a 3-d culture.
社団法人日本生物工学会の論文
2007-03-25