Comparison of solubilization and preparation procedures for membrane-enriched proteome analysis
スポンサーリンク
概要
- 論文の詳細を見る
Proteome analysis of membrane proteins by two-dimentional electrophoresis (2-DE) is still insufficient due to the problem of membrane proteins solubilization. Additionally, nucleic acids and lipids are known to interfere with the profile on 2-DE, and removal of these substances is often required. In this study, three reagents from Bio-X Inc. were used to remove these interfering substances from protein. Prior to loading, samples were treated with the reagents for 30-60 min at 30°C. Pretreatment with the reagents led to high-resolution separations of proteins. Here, we describe how sample pretreatment using the reagents improved proteomic maps of proteins extracted from Escherichia coli and Saccharomyces cerevisiae. To remove lipids enhanced the solubility and separation of membrane proteins from cells.
- 2007-06-01
著者
-
Matsui Hiroshi
Graduate School Of Science Osaka University
-
Nishikawa Tomoko
Graduate School Of Agriculture Kyoto Prefectural University
-
Hirooka Kiyoo
Industrial Technology Center Kyoto Municipal Industrial Research Institute
-
Tsujimoto Yoshiyuki
Division Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Kyoto Prefectur
-
TSUJIMOTO Yoshiyuki
Graduate School of Agriculture, Kyoto Prefectural University
-
TAGUCHI Noritaka
Graduate School of Agriculture, Kyoto Prefectural University
-
TOMARI Naohiro
Industrial Technology Center, Kyoto Municipal Industrial Research Institute
-
OKAMI Akiko
Graduate School of Agriculture, Kyoto Prefectural University
-
NAKAZAWA Yusuke
Graduate School of Agriculture, Kyoto Prefectural University
-
WATANABE Kunihiko
Graduate School of Agriculture, Kyoto Prefectural University
-
YAMAMOTO Yoshihiro
Industrial Technology Center, Kyoto Municipal Industrial Research Institute
-
Okami Akiko
Graduate School Of Agriculture Kyoto Prefectural University
-
Tomari Naohiro
Industrial Technology Center Kyoto Municipal Industrial Research Institute
-
Nakazawa Yusuke
Graduate School Of Agriculture Kyoto Prefectural University
-
Yamamoto Yoshihiro
Industrial Technology Center Kyoto Municipal Industrial Research Institute
-
Taguchi Noritaka
Graduate School Of Agriculture Kyoto Prefectural University
-
Matsui Hiroshi
Division Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Kyoto Prefectur
-
Watanabe Kunihiko
Division Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Kyoto Prefectur
関連論文
- π-d Correlation in λ-(BEDT-TSF)_2Fe_Ga_xCl_4 by ESR Measurements(Condensed matter: electronic structure and electrical, magnetic, and optical properties)
- Split Fermi Surface Properties of LaTGe_3 (T: Transition Metal) and PrCoGe_3 with the Non-centrosymmetric Crystal Structure(Condensed matter: electronic structure and electrical, magnetic, and optical properties)
- de Haas-van Alphen Effect in LaFePO with Two-Dimensional Cylindrical Fermi Surfaces(Condensed matter: electronic structure and electrical, magnetic, and optical properties)
- Magnetic and Superconducting Properties of CeTX_3 (T: Transition Metal and X: Si and Ge) with Non-centrosymmetric Crystal Structure(Condensed matter: electronic structure and electrical, magnetic, and optical properties)
- Infrared spectroscopy on poly(dG)-poly(dC) DNA at low hydration
- IS013 Cellular Basis for the Acute Inhibitory Effects of IL-6-and TNF-α on Excitation-Contraction Coupling
- de Haas-van Alphen Oscillation in Both Normal and Superconducting Mixed States of CeRu_2
- Enhancement of the ^1H NMR Relaxation Rate at Low Temperatures and Development of Antiferromagnetic Spin Fluctuations of π-Electrons in Superconducting λ-(BETS)_2GaCl_4(Condensed Matter : Electronic Structure, Electrical, Magnetic and Optical Properties)
- ^Se NMR Evidence for the Development of Antiferromagnetic Spin Fluctuations of π-Electrons in λ-(BETS)_2GaCl_4
- Gene Expressions of Keratinocyte Growth Factor and Its Receptor in the Human Endometrium/Decidua and Chorionic Villi
- Mother-to-Infant Transmission of Hepatitis C Virus in Human Immunodeficiency Virus-Coinfected Mother : A Case Report
- Continuous Stimulation of Gonadotropin-Releasing Hormone (GnRH) Receptors by GnRH Agonist Decreases Pituitary GnRH Receptor Messenger Ribonucleic Acid Concentration in Immature Female Rats
- Posthemorrhagic Hydrocephalus in a Fetus with Severe Tachycardia
- IS-29 Detection of GnRH mRNA in the human decidua
- Anomalous Splitting of ^1H-NMR Spectra in λ-(BEDT-TSF)_2FeCl_4
- Hydration Effects on the Microwave Dielectricity in Dry Poly(dA)-Poly(dT) DNA(Cross-disciplinary physics and related areas of science and technology)
- Cyclotron Resonance in Extremely Low Carrier-Density System of Yb_4As_3
- Gene Expression Profiles of Prostate Cancer Cell Lines, LNCaP, PC-3 and DU-145, Assessed by cDNA Microarray
- Genomewide linkage analysis of familial prostate cancer in the Japanese population
- Vitamin D receptor gene polymorphism in familial prostate cancer in a Japanese population
- Magnetic Field Effects on Nonlinear Electrical Transport in λ-(BEDT-TSF)_2FeCl_4, where BEDT-TSF is bis(ethylenedithio)tetraselenafulvalene
- Comparison of solubilization and preparation procedures for membrane-enriched proteome analysis
- Acoustic de Hass-van Alphen Effect in Organic Superconductor α-(BEDT-TTF)_2NH_4Hg(SCN)_4
- Magnetic Quantum Oscillations in In_Ga_As/In_Al_As Multiquantum Well Observed by Millimeter Wave Responce
- Transport Properties in InP/InAlAs Type II Single Heterostructure
- Detector Chamber Systems for Measurements of Internal Conversion Electrons Usingan On-line Mass Separator,KUR-ISOL
- Fermi Surface Properties in CeCo_2
- Effect of Amplification of Desensitized purF and prs on Inosine Accumulation in Escherichia coli(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Effects of xapA and guaA Disruption on Inosine Accumulation in Escherichia coli
- Effects of edd and pgi Disruptions on Inosine Accumulation in Escherichia coli
- gsk Disruption Leads to Guanosine Accumulation in Escherichia coli
- Adenine Deaminase Activity of the yicP Gene Product of Escherichia coli
- Investigation of Various Genotype Characteristics for Inosine Accumulation in Escherichia coli W3110
- Familial Prostate Cancer in Four Brothers
- Elastic Anomalies and Acoustic de Haas-van Alphen Effects in Sr_2RuO_4
- Enzymatic degradation of fibroin fiber by a fibroinolytic enzyme of Brevibacillus thermoruber YAS-1(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Molecular Determinants of Substrate Recognition in Thermostable α-glucosidases Belonging to Glycoside Hydrolase Family 13
- Decomposition of Extremely Hard-to-Degrade Animal Proteins by Thermophilic Bacteria
- Potencies of Phosphine Peptide Inhibitors of Mammalian Thimet Oligopeptidase and Neurolysin on Two Bacterial Pz Peptidases
- Proteolytic Activation of Tissue-Type Plasminogen Activator by the Culture Media of Mouse Cancer Cells
- Potencies of Phosphine Peptide Inhibitors of Mammalian Thimet Oligopeptidase and Neurolysin on Two Bacterial Pz Peptidases
- Clinical Observations of Sensory Impairment and Recovery in Trigeminal Nerve Injury after Orthognathic Surgery
- Reentrant Superconductivity of CeRu_2
- Characterization of a NADH : Dichloroindophenol Oxidoreductase from Bacillus subtilis
- Improved Production of Isoamyl Acetate by a Sake Yeast Mutant Resistant to an Isoprenoid Analog and Its Dependence on Alcohol Acetyltransferase Activity, but Not on Isoamyl Alcohol Production (Brewing and Food Technology)
- Comprehensive characterization of secreted aspartic proteases encoded by a virulence gene family in Candida albicans
- Functional roles of YPT31 and YPT32 in clotrimazole resistance of Saccharomyces cerevisiae through effects on vacuoles and ATP-binding cassette transporter(s)(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Measurement of Deposition Fraction of Aerosol Particles in a Horizontal Straight Metal Pipe
- Production of elemental iodine from radioactive methyl iodide under sunlight.
- Calibration of Cascade Impactor by Two-Stage Method