Gene Expression Profiles of Prostate Cancer Cell Lines, LNCaP, PC-3 and DU-145, Assessed by cDNA Microarray
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概要
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Purpose: In the basic research of prostate cancer, prostate cancer cell lines are very valuable tools tocharacterize the biology of prostate cancer. Three cell lines including LNCaP, PC-3 and DU-145 arefrequently utilized. Characterization of molecular characteristics of them is very important. In thepresent study, we screened gene expression patterns of human prostate cancer cell lines, LNCaP, PC-3 andDU-145, using cDNA microarray. Methods: Total RNA from LNCaP, PC-3 or DU-145 cells wasreversely transcribed in the presence of Cy3 or Cy5 as a probe. Mixed probes were hybridized to a slideglass carrying 557 kinds of cancer-related known genes. Signal intensities of Cy-3 and Cy-5 wereseparately scanned, and relative signal ratios of Cy3/Cy5 were analyzed. Gene expression levels ofseveral genes were quantified by real-time PCR. Results: We detected over-expressed or underexpressedgenes in each cell line. Among these genes,farnesyldiphosphate farnesyltransferase 1, amphiregulin,E-cadherin, urokinase-type plasminogen activator (uPA), and glutathione peroxidase 1 geneexpression levels were quantified using quantitative real-time PCR. The relation of the gene expressionpatterns of these genes was consistent with those assessed by real-time PCR. Conclusions: Geneexpression profiles of human prostate cancer cells varied among cell lines. These profiles will provideuseful information on prostate cancer cell lines in the design of experiments, analysis of experimentaldata, and so on.
- 北関東医学会の論文
- 2008-11-01
著者
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Suzuki K
Department Of Urology Gunma University Graduate School Of Medicine
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Suzuki Kazuhiro
Department of Earth and Environmental Sciences, Nagoya University
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Matsui H
Graduate School Of Science Osaka University
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Matsui Hiroshi
Department of Cardiovascular Medicine, Graduate School of Medicine, University of Tokyo
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Suzuki Keita
Environmental Health Sciences Tohoku University Graduate School Of Medicine
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Suzuki Maki
東北大学 医系研究 障害科学 専攻 高次機能障害学 分野
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Minaguchi Hiroshi
Department Of Obstetrics And Gynecology Yokohama City University School Of Medicinedepartment Of Obs
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Okamura Keigo
Department of Urology, Gunma University Graduate School of Medicine
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Koike Hidekazu
Department of Urology, Gunma University Graduate School of Medicine
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Koike Hidekazu
Department Of Urology Gunma University Graduate School Of Medicine
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Koike Hidekazu
Department Of Biology Faculty Of Science Ehime University
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Matsui Hiroshi
Graduate School Of Science Osaka University
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Suzuki Kazuhiro
Department Of Urology Gunma University Graduate School Of Medicine
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Suyama Kazuho
Department Of Urology Kitasato University School Of Medicine
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Suyama Kazuho
Departments Of Urology Kitasato University School Of Medicine
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Okamura Keigo
Department Of Urology Gunma University Graduate School Of Medicine
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Suyama Kazuho
Department Of Urology Shintoshi Clinic
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Matsui Hiroshi
Physics Department Graduate School Of Science Tohoku University:center For Interdisciplinary Researc
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Sentani Kazuhiro
Department Of Molecular Pathology Hiroshima University Graduate School Of Biomedical Sciences
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Muranaka Hiroshi
Graduate School Of Science Osaka University
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Matsui Hiroshi
Physics Department Graduate School Of Science Tohoku University:center For Interdisciplinary Researc
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Suzuki Kazuo
Gunma University
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Suzuki Kazuhiro
Department Of Cardiovascular Surgery Yamaguchi Grand Medical Center
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