Proteolytic Activation of Tissue-Type Plasminogen Activator by the Culture Media of Mouse Cancer Cells
スポンサーリンク
概要
- 論文の詳細を見る
Human single-chain tissue-type plasminogen activator(tPA)was activated by the culture media of mouse B16 melanoma and Lewis lung carcinoma cells. This activation was due to the proteolytic conversion of single-chain tPA to two-chain tPA. Typical serine proteinase inhibitors, such as diisopropylfluorophosphate and aprotinin, strongly inhibited the proteolytic activation, suggesting that the enyzme responsible for this activation is a serine proteinase. Through a process of gel filtration, the molecular weight of the putative tPA-activating enzyme was estimated to be approximately 35 kDa. Expression of the tPA mRNA was demonstrated by Northern blot analysis both for the melanoma and carcinoma cells. Zymographic experiments showed that the culture medium of the melanoma cells contained active two-chain tPA. These results indicate that a common serine proteinase may be involved in the proteolytic activation of single-chain tPA in these cancer cells.
- 社団法人日本動物学会の論文
著者
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Matsui Hitoshi
Division of Biological Sciences, Graduate School of Science, Hokkaido University
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Takahashi Takayuki
Division of Biological Sciences, Graduate School of Science, Hokkaido University
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Ohnishi Junji
Division of Biological Sciences, Graduate School of Science, Hokkaido University
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Takahashi Takayuki
Division Of Biological Sciences Graduate School Of Science Hokkaido University
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Ohnishi Junji
Division Of Biological Science Graduate School Of Science Hokkaido University
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Matsui H
Division Of Biological Sciences Graduate School Of Science Hokkaido University
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Takahashi Takayuki
Division Of Biological Science Graduate School Of Science Hokkaido University
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Ohnishi Junji
北海道大学
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Matsui Hitoshi
Division Of Biological Sciences Graduate School Of Science Hokkaido University
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Matsui Hiroshi
Division Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Kyoto Prefectur
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