Expression and Localization of Prolyl Oligopeptidase in Mouse Testis and Its Possible Involvement in Sperm Motility(Reproductive Biology)
スポンサーリンク
概要
- 論文の詳細を見る
Prolyl oligopeptidase (POP) expression in mouse testis during sexual maturation was examined. Northern blot analysis showed that POP mRNA expression was highest at 2 weeks of age, and gradually reduced thereafter. However, enzyme activity was almost constant during the examined period. In situ hybridization study revealed a change in the expression site of POP mRNA in testis during sexual maturation. Positive signals were detected in all types of cells in the seminiferous tubules before maturation, and were restricted to spermatids at the spermatogenesis cycle stages I-VIII in adult mice. POP was detected in the insoluble fraction of sperm by Western blot analysis. Immunohistochemical analyses showed that POP is localized in the spermatids at steps 12-16 of spermiogenesis and in the midpiece of the sperm fragellum. It was also found that specific POP inhibitors, poststatin and benzyloxycarbonyl-proline-prolinal, suppressed sperm motility. These results suggest that POP may be involved in meiosis of spermatocytes, differentiation of spermatids, and sperm motility in the mouse.
- 社団法人日本動物学会の論文
- 2002-01-25
著者
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高橋 孝行
北海道大学大学院先端生命科学研究院
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高橋 孝行
Dep. Of Biophysics And Biochemistry Graduate School Of Sci. Univ. Of Tokyo
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Kimura Atsushi
Division of Biological Sciences, Graduate School of Science, Hokkaido University
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Matsui Hitoshi
Division of Biological Sciences, Graduate School of Science, Hokkaido University
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Takahashi Takayuki
Division of Biological Sciences, Graduate School of Science, Hokkaido University
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Kimura Atsushi
Laboratory Of Molecular And Cellular Interactions Faculty Of Advanced Life Science Hokkaido Universi
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Matsui H
Division Of Biological Sciences Graduate School Of Science Hokkaido University
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Takahashi Takayuki
Division Of Biological Science Graduate School Of Science Hokkaido University
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Matsui Hitoshi
Division Of Biological Sciences Graduate School Of Science Hokkaido University
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Takahashi Takayuki
Department Of Biophysics And Biochemistry Graduate School Of Science University Of Tokyo
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Kimura Atsushi
Division Of Biological Sciences Graduate School Of Life Science Hokkaido University
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