Analysis of Viral Infection by Multiplex Polymerase Chain Reaction Assays in Patients with Liver Dysfunction
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概要
- 論文の詳細を見る
Objective While unexplained liver dysfunction is common, it is sometimes difficult to identify its exact cause. One cause is viral infections. The identification of viruses other than hepatitis B and C that cause liver dysfunction is difficult because no methods to simultaneously identify these viruses have been established. The aim of this study was to quickly and simultaneously identify multiple virus species. Methods A total of 49 patients with unexplained liver dysfunction and undetermined inflammation were examined. The majority of patients had hematologic malignancies, and some had undergone bone marrow transplantation. Qualitative polymerase chain reactions (PCR) were performed to detect 12 species of DNA virus in whole blood. Quantitative real-time PCR was performed when a specific virus was amplified. In addition, 6 RNA hepatitis viruses were directly assayed by real-time PCR. These 2 PCR steps were completed within 1 hour. Results The most frequently detected virus in 37 patients with liver dysfunction, was transfusion transmitted virus (38%), which was followed by human herpes virus (HHV) type 6 (35%), Epstein-Barr virus (14%), cytomegalovirus (8%), and rarely hepatitis G virus and HHV-7 (3%). Similar viremia was observed in 12 patients with mild liver dysfunction. The results of the PCR assay were mostly consistent with those of routine virus serological tests. Conclusion A multiplex viral PCR assay was a useful tool for quickly identifying viruses that possibly cause liver dysfunction. It was also important that liver dysfunction acted as a proband that led to the discovery of serious viremia.
著者
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Ito Kiminari
Department Of Cell Therapy Institute Of Biomedical Research And Innovation
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Akasaka Hiroshi
Department Of Internal Medicine Ii Sapporo Medical University
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Tsunemine Hiroko
Department Of Hematology And Oncology Kyoto University Hospital
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Shimizu Norio
Department Of Surgery Tottori University School Of Medicine
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Takahashi Takayuki
Department Of Biophysics And Biochemistry Graduate School Of Science University Of Tokyo
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Watanabe Ken
Department Of Bone And Joint Disease Research Institute National Center For Geriatrics And Gerontology (ncgg)
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Kodaka Taiichi
Department of Hematology, Shinko Hospital
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Saito Toshiharu
Laboratory of Cell Therapy, Shinko Hospital, Japan
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Yoshioka Yuriko
Laboratory of Cell Therapy, Shinko Hospital, Japan
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Sakane Emiko
Department of Hematology, Shinko Hospital, Japan
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Akasaka Hiroshi
Department of Hematology, Shinko Hospital
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Ito Kiminari
Department of Hematology, Shinko Hospital, Japan
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