Development of an Improved Assay for Purine Nucleoside Kinase Activity in Cell Extracts and Detection of Inosine Kinase Activity in Brevibacterium acetylicum ATCC 953,Related Species, and Corynebacterium flaccumfaciens ATCC 6887
スポンサーリンク
概要
- 論文の詳細を見る
An improved assay was developed to detect direct purine nucleoside phosphorylating activity in cell-free extracts. Direct inosine phosphorylating activity was detected in 2 of 70 species tested. Both activities, which depended on magnesium ion and ATP, phosphorylated a hydroxyl group at the 5' position of inosine. The new assay was shown to be useful for screening of direct purine nucleoside phosphorylating activity and have the potential to detect inosine kinase in the presence of a background of nucleoside phosphorylase and purine phosphoribosyltransferase activities. Previously, the latter two activities made it difficult to correctly detect direct phosphorylation of inosine by inosine kinase.
- 社団法人日本農芸化学会の論文
- 2000-04-23
著者
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Kawasaki Haruhiko
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture University
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Kawasaki Haruhiko
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture Univeisity
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Kawasaki Haruhiko
Department Of Applied Biochemistry Faculty Of Agriculture University Of Osaka Prefecture
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Kawasaki H
Department Of Environmental Materials Science Tokyo Denki University
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Shimaoka Megumi
Fermentation and Biotechnology Laboratories, Ajinomoto Co., Inc.
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Shimaoka M
Fermentation And Biotechnology Laboratories Ajinomoto Co. Inc.
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KAWASAKI Hisashi
Applied Microbiology Laboratory, Fermentation and Biotechnology Laboratories, Ajinomoto Co. Inc.
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USUDA Yoshihiro
Applied Microbiology Laboratory, Fermentation and Biotechnology Laboratories, Ajinomoto Co. Inc.
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SHIMAOKA Megumi
Applied Microbiology Laboratory, Fermentation and Biotechnology Laboratories, Ajinomoto Co. Inc.
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UTAGAWA Takashi
Applied Microbiology Laboratory, Fermentation and Biotechnology Laboratories, Ajinomoto Co. Inc.
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Utagawa T
Ajinomoto Co. Inc. Kawasaki‐shi Jpn
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Utagawa Takashi
Applied Microbiology Laboratory Fermentation And Biotechnology Laboratories Ajinomoto Co. Inc.
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Usuda Yoshihiro
Applied Microbiology Laboratory Fermentation And Biotechnology Laboratories Ajinomoto Co. Inc.
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