Introduction of Enterostatin (VPDPR) and a Related Sequence into Soybean Proglycinin A_<1a>B_<1b> Subunit by Site-directed Mutagenesis
スポンサーリンク
概要
- 論文の詳細を見る
Enterostatin (VPDPR), having anoretic and hypocholesterolemic activities, and its homologue LPYPR, a hypocholesterolemic peptide found in the glycinin A_5A_4B_3 subunit, were introduced into the corresponding site (TNGPQ) of the proglycinin A_<1a>B_<1b> subunit by site-directed mutagenesis. Modified proglycinins were expressed in E. coli and recovered from the insoluble fraction. VPDPR and LPYPR were released by the action of chymotrypsin and trypsin as expected. The overall yields of purified VPDPR and LPYPR were 40% and 62%, respectively.
- 社団法人日本農芸化学会の論文
- 2000-12-23
著者
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UTSUMI Shigeru
Research Institute for Food Science, Kyoto University
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YOSHIKAWA Masaaki
Research Institute for Food Science, Kyoto University
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TAKENAKA Yasuyuki
Research Institute for Food Science, Kyoto University
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YOSHIKAWA Masaaki
Graduate School of Agriculture, Kyoto University
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TAKENAKA Yasuyuki
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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Yoshikawa Masaaki
Research Institute For Food Science Kyoto University
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Utsumi S
Division Of Agronomy And Horticultural Science Graduate School Of Agriculture Kyoto University
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Utsumi Shigeru
Research Institute For Food Science Kyoto University
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Takenaka Y
Fermentation And Biotechnology Laboratories Ajinomoto Co. Inc.
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Yoshikawa Masaaki
Kyoto Univ. Kyoto Jpn
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Takenaka Y
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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Yoshikawa M
Graduate School Of Agriculture Kyoto University
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Takenaka Yasuyuki
Kobe Shoin Woman's University
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