Gallic acid oxidizes Met residues in peptides released from bovine β-lactoglobulin by in vitro digestion(BREWING AND FOOD TECHNOLOGY)
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概要
- 論文の詳細を見る
Phenolic compounds (PCs) are frequently present in foods. However, little is known about the effect of PCs on enzymatic digestion process of food proteins and their products. In this study, the effect of gallic acid (GA) on in vitro digestion of β-lactoglobulin (β-LG) was investigated as a model system for analysis of the interaction between PCs and food proteins. GA showed no effect on the initial rate of β-LG digestion. However, after 1.5 h of digestion, the observed degree of hydrolysis of β-LG was lower in the presence than in the absence of GA. The peptides released from β-LG were characterized by LC/IT-TOF-MS and thirty peptides were identified. In particular, four new peaks were obtained following in vitro digestion of β-LG in the presence of GA. Met^7, Met^<24> and Met145^<145> in the peptides corresponding to these peaks were oxidized to methionine sulfoxide residues.
著者
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FUKUSAKI EI-ICHIRO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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KAWABE Akira
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Kobayashi A
National Inst. Advanced Industrial Sci. And Technol. Ibaraki Jpn
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Bamba Takeshi
Department Of Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka Univers
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Kobayashi Akio
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.
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Kobayashi A
Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Kobayashi A
Ochanomizu Univ. Tokyo Jpn
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Kobayashi A
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.
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Kawabe Akira
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Bamba Takeshi
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ. 2- 1 Yamadaoka Suita Osaka 565-0871
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Bamba Takeshi
Dept. Biotech. Grad. Sch. Eng. Osaka Univ.:hitachi Zosen Co.
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Yoshikawa Masaaki
Research Institute For Food Science Kyoto University
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Fukusaki Eiichiro
Division Of Advanced Science And Biotechnology Graduate School Of Engineering Osaka University
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Fukusaki Eiichiro
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.
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Fukusaki Ei-ichiro
大阪大学大学院
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Fukusaki Eiichiro
Department Of Biotechnology Faculty Of Engineering Osaka University
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Fukusaki Eiichiro
Dept. Mat. Life Sci. Osaka Univ
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Okazawa Atsushi
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.
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Okazawa Atsushi
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Okazawa Atsushi
Department Of Applied Physics And Chemistry The University Of Electro-communications
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Izumi Yoshihiro
Dept. Biotech. Grad. Sch. Eng. Osaka Univ.
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Izumi Yoshihiro
Department Of Biological Science Faculty Of Science Kumamoto University
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Lai Ping
Dept. Biotech. Grad. Sch. Eng. Osaka Univ.
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Yoshikawa Masaaki
Research Institute For Production Development
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Yoshikawa Masaaki
Dept. Biotech. Grad. Sch. Eng. Osaka Univ.
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Lai Ping
Department Of Physical Therapy And Rehabilitation Da Chien General Hospital
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Kobayashi Akio
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Lai Ping
Department Of Biotechnology Graduate School Of Engineering Osaka University
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KOBAYASHI Akio
Department of Biochemistry, Shinshu University School of Medicine
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KOBAYASHI Akio
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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