High Throughput and Exhaustive Analysis of Diverse Lipids by Using Supercritical Fluid Chromatography-Mass Spectrometry for Metabolomics(METHODS)
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概要
- 論文の詳細を見る
We have developed an analytical system that enables the simultaneous rapid analysis of lipids with varied structures and polarities through the use of supercritical fluid chromatography-mass spectrometry(SFC-MS). The separation conditions for SFC(column, modifier, back pressure, etc.) and the detection conditions for mass spectrometry(ionization method, parameters, etc.) were investigated to develop a simultaneous analytical method for lipid mixtures that included phospholipids, glycolipids, neutral lipids, and sphingolipids. When cyanopropylated silica gel-packed column was used for the separation, all lipids were successfully detected and the analysis time was less than 15 min. The use of an octadecylsilylated column resulted in separation, which was dependent on the differences in the unsaturation of the fatty acid side chains and isomer separation. This system is a powerful tool for studies on lipid metabolomics because it is useful not only as a finger- printing method for the screening of diverse lipids but also for the detailed profiling of individual components.
- 社団法人日本生物工学会の論文
- 2008-05-25
著者
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Bamba Takeshi
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Fukusaki Eiichiro
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Harada Kazuo
Department Of Biotechnology Graduate School Of Engineering Osaka University:(present Office)departme
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FUKUSAKI EI-ICHIRO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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KOBAYASHI AKIO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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KAWABE Akira
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Hirata Kazumasa
Department of Applied Environmental Biology, Graduate School of Pharmaceutical Sciences, Osaka Unive
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Kobayashi A
National Inst. Advanced Industrial Sci. And Technol. Ibaraki Jpn
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Bamba Takeshi
Department Of Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka Univers
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Kobayashi Akio
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.
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Kobayashi A
Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Harada Kazuo
Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka University
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Kobayashi A
Ochanomizu Univ. Tokyo Jpn
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Kobayashi A
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.
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NAKAZAWA Yoshihisa
Hitachi Zosen Corporation
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Kawabe Akira
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Bamba Takeshi
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ. 2- 1 Yamadaoka Suita Osaka 565-0871
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Bamba Takeshi
Dept. Biotech. Grad. Sch. Eng. Osaka Univ.:hitachi Zosen Co.
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Shimonishi Naruto
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Matsubara Atsuki
Department of Applied Environmental Biology, Graduate School of Pharmaceutical Sciences, Osaka Unive
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Nakazawa Yoshihisa
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Nakazawa Yoshihisa
Hitachi Zosen Co.
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Fukusaki Eiichiro
Division Of Advanced Science And Biotechnology Graduate School Of Engineering Osaka University
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Fukusaki Eiichiro
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.
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Fukusaki Ei-ichiro
大阪大学大学院
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Hirata Kazumasa
Dep. Of Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Fukusaki E
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Fukusaki Eiichiro
Department Of Biotechnology Faculty Of Engineering Osaka University
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Fukusaki Eiichiro
Dept. Mat. Life Sci. Osaka Univ
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Matubara Atuki
Dept. Mat. Life Sci. Osaka Univ
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Harada Kazuo
Department Of Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka Univers
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Matsubara Atsuki
Department Of Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka Univers
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Shimonishi Naruto
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kobayashi Akio
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Nishiumi Shin
Division Of Gastroenterology Kobe University Graduate School Of Medicine
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KOBAYASHI Akio
Department of Biochemistry, Shinshu University School of Medicine
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KOBAYASHI Akio
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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