A Transient RNA Interference Assay System Using Arabidopsis Protoplasts(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using protoplasts from Arabidopsis mesophyll cells and suspension-cultured cells (cell line T87). Introduction of dsRNA into protoplasts led to marked silencing of target transgenes. Our assay system would provide a convenient and efficient way to induce RNAi in protoplasts of the model plant Arabidopsis thaliana.
- 社団法人日本農芸化学会の論文
- 2003-12-23
著者
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An Chung-il
Department Of Biotechnology Graduate School Of Engineering Osaka University
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FUKUSAKI EI-ICHIRO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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KOBAYASHI AKIO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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KAWABE Akira
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Kobayashi A
National Inst. Advanced Industrial Sci. And Technol. Ibaraki Jpn
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Kobayashi A
Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Kobayashi A
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.
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SAWADA Aki
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Fukusaki Ei-ichiro
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Fukusaki Eiichiro
Division Of Advanced Science And Biotechnology Graduate School Of Engineering Osaka University
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Fukusaki Ei-ichiro
大阪大学大学院
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Sawada A
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Fukusaki E
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Sawada Aki
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kobayashi Akio
Department Of Biotechnology Graduate School Of Engineering Osaka University
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KOBAYASHI Akio
Department of Biochemistry, Shinshu University School of Medicine
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KOBAYASHI Akio
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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