Simultaneous quantification of lignans in Arabidopsis thaliana by highly sensitive capillary liquid chromatography-electrospray ionization-ion trap mass spectrometry

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概要

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• Lignans are phenylpropanoid dimers in which the monomers are linked by the central carbon (C8) atoms. Because many lignans have physiological effects, including antioxidant activity, they are now in demand as components in health foods. However, the lignan biosynthetic pathways in plants are only now being understood. Recently, lariciresinol was detected in Arabidopsis thaliana. This observation indicated the existence of common lignan biosynthetic pathways in A. thaliana, despite a low amount of lignans other than lariciresinol glycosides. In this study, we established a highly sensitive analytical method that enables quantification of both glycoside and aglycone forms of lignans in A. thaliana simultaneously using capillary liquid chromatography-electrospray ionization-ion trap mass spectrometry. Some lignans not previously detected in A. thaliana were quantified in extracts of both roots and shoots. Our method can be used for the comprehensive analysis of lignans in small samples from mutants and transformants. This method will be utilized to elucidate the metabolic pathways and physiological roles of lignans as well as the regulation of their biosynthesis in plants.

• 2011-06-25

著者

• SATAKE Honoo

  Suntory Institute for Bioorganic Research

• FUKUSAKI EI-ICHIRO

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• KAWABE Akira

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Kobayashi A

  National Inst. Advanced Industrial Sci. And Technol. Ibaraki Jpn

• Kobayashi Akio

  Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.

• Kobayashi A

  Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Kobayashi A

  Ochanomizu Univ. Tokyo Jpn

• Kobayashi A

  Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.

• Kawabe Akira

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Bamba Takeshi

  Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ. 2- 1 Yamadaoka Suita Osaka 565-0871

• Bamba Takeshi

  Dept. Biotech. Grad. Sch. Eng. Osaka Univ.:hitachi Zosen Co.

• Fukusaki Eiichiro

  Division Of Advanced Science And Biotechnology Graduate School Of Engineering Osaka University

• Fukusaki Eiichiro

  Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.

• Fukusaki Ei-ichiro

  大阪大学大学院

• Ono Eiichiro

  Core Research Group R&d Planning Division Suntory Holdings Ltd. Suntory Research Center

• Fukusaki E

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Fukusaki Eiichiro

  Dept. Mat. Life Sci. Osaka Univ

• Okazawa Atsushi

  Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.

• Okazawa Atsushi

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Izumi Yoshihiro

  Dept. Biotech. Grad. Sch. Eng. Osaka Univ.

• Okazawa Atsushi

  Graduate School of Engineering, Osaka University

• Hori Katsuhito

  Graduate School of Engineering, Osaka University

• Okumura Ryohei

  Graduate School of Engineering, Osaka University

• Izumi Yoshihiro

  Graduate School of Engineering, Osaka University

• Bamba Takeshi

  Graduate School of Engineering, Osaka University

• Fukusaki Eiichiro

  Graduate School of Engineering, Osaka University

• Kobayashi Akio

  Graduate School of Engineering, Osaka University

• Hata Naoki

  Graduate School Of Engineering Osaka University

• ONO Eiichiro

  R & D Planning Division, Suntory Holdings, Ltd.

• Hori Katsuhito

  Graduate School Of Engineering Osaka Univ.

• Bamba Takeshi

  Graduate School Of Engineering Osaka University

• Izumi Yoshihiro

  Graduate School Of Engineering Osaka University

• Okumura Ryohei

  Graduate School Of Engineering Osaka University

• Hori Katsuhito

  Graduate School Of Engineering Osaka University

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