Highly sensitive and rapid profiling method for carotenoids and their epoxidized products using supercritical fluid chromatography coupled with electrospray ionization-triple quadrupole mass spectrometry(METHODS)
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概要
- 論文の詳細を見る
Epoxy carotenoids, which are products of carotenoid oxidation, are potential oxidative stress markers. However, it is difficult to profile epoxy carotenoids owing to their small amount and difficulty in their separation from hydroxy carotenoids. In this study, a high-performance analytical system based on supercritical fluid chromatography (SFC) coupled with tandem mass spectrometry (MS/MS) was developed for the simultaneous analysis of carotenoids and epoxy carotenoids. SFC is an effective separation technique for hydrophobic compounds, by which major carotenoids in human serum and their epoxidation products can be analyzed within 20 min. The use of MS/MS increased the sensitivity; the detection limit for each carotenoid was of the sub-fmol order. When the constructed method was applied to biological samples such as human serum and low-density lipoprotein (LDL), the precise detection of the target carotenoids was disturbed by several isomers. However, highly selective detection of epoxy carotenoids was performed by targeting product ions that were generated with a structure-specific neutral loss of 80 Da. Furthermore, the sample volume needed for the analysis was only 0.1 ml for the serum, indicating the efficiency of this system in performing small-scale analyses. Using the analytical system developed in this study, highly sensitive and selective analysis of epoxy carotenoids could be performed in a short time. These features show the usefulness of this system in application to screening analysis of carotenoid profiles that are easily modified by oxidative stress.
著者
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FUKUSAKI EI-ICHIRO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Bamba Takeshi
Dept. Mat. Life Sci. Osaka Univ
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Bamba Takeshi
Department Of Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka Univers
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Yoshida Masaru
Department Of Gastroenterology Kobe University Hospital
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Nishiumi Shin
Division Of Applied Chemistry In Bioscience Department Of Agrobioscience Graduate School Of Agricult
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Fukusaki Eiichiro
Division Of Advanced Science And Biotechnology Graduate School Of Engineering Osaka University
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Fukusaki Eiichiro
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.
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Fukusaki Ei-ichiro
大阪大学大学院
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Fukusaki Eiichiro
Department Of Biotechnology Faculty Of Engineering Osaka University
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Fukusaki Eiichiro
Dept. Mat. Life Sci. Osaka Univ
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Matubara Atuki
Dept. Mat. Life Sci. Osaka Univ
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Yoshida Masaru
Department Of Gastroenterology Kobe University School Of Medicine
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Matsubara Atsuki
Department Of Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka Univers
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Shimonishi Naruto
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Shinohara Masakazu
The Integrated Center For Mass Spectrometry Kobe University Graduate School Of Medicine
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Yoshida Masaru
The Integrated Center for Mass Spectrometry, Kobe University Graduate School of Medicine
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Nishiumi Shin
Division Of Gastroenterology Kobe University Graduate School Of Medicine
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Yoshida Masaru
The Integrated Center For Mass Spectrometry Kobe University Graduate School Of Medicine:division Of
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Uchikata Takato
Dept. Mat. Life Sci. Osaka Univ
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Uchikata Takato
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Uchikata Takato
Department Of Biotechnology Graduate School Of Engineering Osaka University
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