Comprehensive metabolite profiling of phyA phyB phyC triple mutants to reveal their associated metabolic phenotype in rice leaves(PLANT BIOTECHNOLOGY)

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概要

• 論文の詳細を見る

• The phytochrome photoreceptors regulate plant growth and development throughout their life cycle. Rice (Oryza sativa) possesses three phytochromes, phyA, phyB, and phyC. Physiological, genetic, and biochemical analyses of null mutants of each phytochrome have revealed the function of each in rice. However, few studies have investigated the relationship between phytochrome signaling and metabolism. In the present study, non-targeted metabolite analysis by gas chromatography time-of-flight mass spectrometry (GC/TOF-MS) and targeted metabolite analysis by capillary electrophoresis electrospray ionization mass spectrometry (CE/ESI-MS) were employed to investigate metabolic changes in rice phyA phyB phyC triple mutants. Distinct metabolic profiles between phyA phyB phyC triple mutants and the wild type (WT), as well as those between young and mature leaf blades, could be clearly observed by principal component analysis (PCA). The metabolite profiles indicated high accumulation of amino acids, organic acids, sugars, sugar phosphates, and nucleotides in the leaf blades of phyA phyB phyC triple mutants, especially in the young leaves, compared with those in the WT. Remarkable overaccumulation of monosaccharide, such as glucose (53.4-fold), fructose (42.5-fold), and galactose (24.5-fold), was observed in young leaves of phyA phyB phyC triple mutants. These metabolic phenotypes suggest that sugar metabolism, carbon partitioning, sugar transport, or some combination of these is impaired in the phyA phyB phyC triple mutants, and conversely, that phytochromes have crucial roles in sugar metabolism.

• 社団法人日本生物工学会の論文

著者

• Fukusaki Eiichiro

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Takano Makoto

  Department of Physiology and Biophysics, Kyoto University Graduate School of Medicine

• Harada Kazuo

  Department Of Biotechnology Graduate School Of Engineering Osaka University:(present Office)departme

• JUMTEE Kanokwan

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• FUKUSAKI EI-ICHIRO

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• KOBAYASHI AKIO

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• KAWABE Akira

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Kobayashi A

  National Inst. Advanced Industrial Sci. And Technol. Ibaraki Jpn

• Kobayashi Akio

  Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.

• Kobayashi A

  Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• OKAZAWA Atsushi

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Harada Kazuo

  Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka University

• Kobayashi A

  Ochanomizu Univ. Tokyo Jpn

• Takano Makoto

  Department Of Cardiology Tokyo Metropolitan Hiroo General Hospital

• Kobayashi A

  Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.

• Kawabe Akira

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Jumtee Kanokwan

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Fukusaki Eiichiro

  Division Of Advanced Science And Biotechnology Graduate School Of Engineering Osaka University

• Fukusaki Eiichiro

  Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.

• Fukusaki Ei-ichiro

  大阪大学大学院

• Hirata Kazumasa

  Dep. Of Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka Univ.

• Fukusaki E

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Fukusaki Eiichiro

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Fukusaki Eiichiro

  Dept. Mat. Life Sci. Osaka Univ

• Okazawa Atsushi

  Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.

• Okazawa Atsushi

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Okazawa Atsushi

  Department Of Applied Physics And Chemistry The University Of Electro-communications

• Harada Kazuo

  Department Of Applied Environmental Biology Graduate School Of Pharmaceutical Sciences Osaka Univers

• Takano Makoto

  Department Of Plant Physiology National Institute Of Agrobiological Sciences

• Jumtee Kanokwan

  Nara Prefectural Small And Medium Sized Enterprises Support Corporation:department Of Biotechnology

• Kobayashi Akio

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• KOBAYASHI Akio

  Department of Biochemistry, Shinshu University School of Medicine

• KOBAYASHI Akio

  Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University

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