Cloning and Characterization of Mevalonate Pathway Genes in a Natural Rubber Producing Plant, Hevea brasiliensis
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概要
- 論文の詳細を見る
Hevea brasiliensis Müll. Arg. is a tree that produces natural rubber, an industrially vital isoprenoid polymer. Biosynthesis of natural rubber is known to take place biochemically by a mevalonate (MVA) pathway, but molecular biological characterization of related genes has been insufficient. From H. brasiliensis, we obtained full-length cDNA of genes encoding all of the enzymes that catalyze the six steps of the MVA pathway. Alignment analysis and phylogenetic analysis revealed that in H. brasiliensis there are three acetyl-CoA acetyltransferase genes, two HMG-CoA synthase (HMGS) genes, and four HMG-CoA reductase (HMGR) genes. Gene expression analysis by type of tissue indicated that MVA pathway genes were highly expressed in latex, as compared to other types of tissue and that HMGS and HMGR, which exist in multiple copies, have different expression patterns. Moreover, these MVA pathway genes in H. brasiliensis were found to complement MVA pathway deletion mutations in yeast.
- 社団法人 日本農芸化学会の論文
- 2008-08-23
著者
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Fukusaki Eiichiro
Department of Biotechnology, Graduate School of Engineering, Osaka University
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FUKUSAKI EI-ICHIRO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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KOBAYASHI AKIO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Kobayashi Akio
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.
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Kobayashi A
Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Fukusaki Eiichiro
Division Of Advanced Science And Biotechnology Graduate School Of Engineering Osaka University
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Fukusaki Eiichiro
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.
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Fukusaki Ei-ichiro
大阪大学大学院
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MUKAI YUKIO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Fukusaki E
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Fukusaki Eiichiro
Department Of Biotechnology Faculty Of Engineering Osaka University
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Fukusaki Eiichiro
Dept. Mat. Life Sci. Osaka Univ
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Hattori Masahira
Graduate School Of Frontier Sciences The Univ. Of Tokyo
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YAMASHITA Atsushi
Kitasato Institute for Life Sciences, Kitasato University
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TAKAOKA Chika
Department of Biological Science, Faculty of Science, Kumamoto University
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Mukai Yukio
Department Of Bioscience Faculty Of Bioscience Nagahama Institute Of Bio-science And Technology
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SANDO Tomoki
Department of Biotechnology, Graduate School of Engineering, Osaka University
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OGASAWARA Naotake
Department of Bioinformatics and Genomics, Graduate School of Information Science, Nara Institute of
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Takaoka Chika
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Takaoka Chika
Department Of Biological Science Faculty Of Science Kumamoto University
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Sando Tomoki
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Ogasawara Naotake
Graduate School Of Information Sciences Nara Institute Of Science And Technology
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Hattori Masahira
Graduate School Of Frontier Sciences The University Of Tokyo
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Yamashita Atsushi
Kitasato Institute For Life Sciences Kitasato University
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Kobayashi Akio
Department Of Biotechnology Graduate School Of Engineering Osaka University
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KOBAYASHI Akio
Department of Biochemistry, Shinshu University School of Medicine
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KOBAYASHI Akio
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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