Overexpression of 1-Deoxy-D-Xylulose-5-Phosphate Reductoisomerase Gene in Chloroplast Contributes to Increment of Isoprenoid Production(PLANT BIOTECHNOLOGY)
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概要
- 論文の詳細を見る
Plants synthesize a large number of isoprenoid compounds that are of industrial, nutritional and medicinal importance. 1-Deoxy-D-xylulose reductoisomerase(DXR) catalyzes the first committed step of plastidial isoprenoid-precursor biosynthesis. In the present study, we generated transplastomic tobacco plants that overproduced DXR from Synechosystis sp. strain PCC6803. The transformants showed increase in the content of various isoprenoids such as chlorophyll α, β-carotene, lutein, antheraxanthin, solanesol and β-sitosterol, indicating that the DXR reaction is one of the key steps controlling isoprenoid level in tobacco leaves. A qualitative change in isoprenoid composition was also observed. The growth phenotype of the transplastomic plants was similar to that of wild-type plants. These results showed that plastid metabolic engineering is useful in manipulating the yield of isoprenoids in plants.
- 社団法人日本生物工学会の論文
- 2008-05-25
著者
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FUKUSAKI EI-ICHIRO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Bamba Takeshi
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ. 2- 1 Yamadaoka Suita Osaka 565-0871
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Bamba Takeshi
Dept. Biotech. Grad. Sch. Eng. Osaka Univ.:hitachi Zosen Co.
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Hasunuma Tomohisa
Research Institute of Innovative Technology for the Earth(RITE)
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Takeno Shinya
Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University
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Hayashi Shunsuke
Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University
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Sendai Mayumi
Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University
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Bamba Takeshi
Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University
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Yoshimura Satomi
Research Institute of Innovative Technology for the Earth(RITE)
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Tomizawa Ken-ichi
Research Institute of Innovative Technology for the Earth(RITE)
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Fukusaki Eiichiro
Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University
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Miyake Chikahiro
Research Institute of Innovative Technology for the Earth(RITE)
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TAKENO Shinya
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Miyake Chikahiro
Research Institute Of Innovative Technology For The Earth (rite)
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Tomizawa Ken-ichi
Research Institute Of Innovative Technology For The Earth (rite)
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Fukusaki Eiichiro
Division Of Advanced Science And Biotechnology Graduate School Of Engineering Osaka University
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Fukusaki Eiichiro
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.
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Fukusaki Ei-ichiro
大阪大学大学院
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Fukusaki E
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Fukusaki Eiichiro
Dept. Mat. Life Sci. Osaka Univ
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Hayashi Shunsuke
Division Of Advanced Science And Biotechnology Graduate School Of Engineering Osaka University
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Sendai Mayumi
Division Of Advanced Science And Biotechnology Graduate School Of Engineering Osaka University
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Takeno Shinya
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Hasunuma Tomohisa
Research Institute Of Innovative Technology For The Earth (rite)
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TOMIZAWA Ken-ichi
Research Institute of Innovative Technology for the Earth
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