Late Type Apoptosis and Apoptosis Free Lethal Effect of Quercetin in Human Melanoma
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概要
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No satisfactory treatment is currently available for metastatic malignant melanoma. Recently, the flavonoid quercetin was suggested as a potential treatment due to its anti-tumorogenic properties. Some of these properties appeared to correspond to those published for UVB irradiation. To determine quercetin’s long-term effects, type of apoptosis, and shared properties with UVB, we exposed Mel-Juso, M14, and G361 human melanoma cell-lines to a large range of quercetin or UVB doses, 20–400 μm and 25–1000 mJ/cm2 respectively. Apoptosis was measured for 4 consecutive d by flow cytometry and cell viability was studied by colony-forming assay. Quercetin decreased cell viability level in a dose-dependent manner to almost zero at 100 μm. Up to this concentration, it did not induce significant apoptosis nor did it decrease the survival-fractions below 90% during a 4 d follow-up. The data suggest that Quercetin is lethal to melanoma cells at concentrations that do not activate apoptosis during the first 4 d post-exposure and that quercetin’s effects extend beyond the period of direct contact. Both quercetin and UVB induced late-type apoptosis at the upper range of the tested doses, but they do not appear to share all the pathways that they activate. Finally, this paper provides novel data showing that quercetin causes two different lethal effects on human melanoma cells, suggesting the activation of at least two different dose-depended mechanisms.
- 社団法人 日本農芸化学会の論文
- 2007-09-23
著者
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Tomizawa Ken-ichi
Research Institute of Innovative Technology for the Earth(RITE)
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Tomizawa Ken-ichi
Research Institute Of Innovative Technology For The Earth (rite)
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Ropke Carsten
Department Of Medical Anatomy The Panum Institute University Of Copenhagen
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ROSNER Karli
Laboratory for Molecular Dermatology, Department of Dermatology, Bispebjerg Hospital, University of
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PLESS Vibeke
Laboratory for Molecular Dermatology, Department of Dermatology, Bispebjerg Hospital, University of
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SKOVGAARD Gunhild
Laboratory for Molecular Dermatology, Department of Dermatology, Bispebjerg Hospital, University of
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Skovgaard Gunhild
Laboratory For Molecular Dermatology Department Of Dermatology Bispebjerg Hospital University Of Cop
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Pless Vibeke
Laboratory For Molecular Dermatology Department Of Dermatology Bispebjerg Hospital University Of Cop
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Rosner Karli
Laboratory For Molecular Dermatology Department Of Dermatology Bispebjerg Hospital University Of Cop
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ADACHI TAKASHI
Research Center, Taisho Pharmaceutical Co. Ltd.,
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TAKASE Hisabumi
Research Institute of Innovative Technology for the Earth (RITE)
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Adachi Takashi
Research Center Taisho Pharmaceutical Co. Ltd.
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Rosner Karli
Research Institute of Innovative Technology for the Earth (RITE)
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Ropke Carsten
Research Institute of Innovative Technology for the Earth (RITE)
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Pless Vibeke
Research Institute of Innovative Technology for the Earth (RITE)
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