Introduction of a 50 kbp DNA Fragment into the Plastid Genome
スポンサーリンク
概要
- 論文の詳細を見る
Plastid transformation technology has been used for the analysis and improvement of plastid metabolism. To create a transplastomic plant with a complicated and massive metabolic pathway, it is necessary to introduce a large amount of DNA into the plastid. However, to our knowledge, the largest DNA fragment introduced into a plastid genome was only 7 kbp long and consisted of just three genes. Here we report the introduction of foreign DNA of 23–50 kbp into the tobacco plastid genome with a bacterial artificial chromosome (BAC)-based plastid transformation vector. It was confirmed that the introduced DNA was passed on to the next generation. This is the first description of plastid transformation with a large amount of foreign DNA.
- 社団法人 日本農芸化学会の論文
著者
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Tomizawa Ken-ichi
Research Institute Of Innovative Technology For The Earth (rite)
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ADACHI TAKASHI
Research Center, Taisho Pharmaceutical Co. Ltd.,
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TAKASE Hisabumi
Research Institute of Innovative Technology for the Earth (RITE)
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Adachi Takashi
Research Institute Of Innovative Technology For The Earth (rite)
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Adachi Takashi
Research Center Taisho Pharmaceutical Co. Ltd.
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TOMIZAWA Ken-ichi
Research Institute of Innovative Technology for the Earth
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