Novel plant transformation system by gene-coated gold particle introduction into specific cell using ArF excimer laser
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概要
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Novel methods for foreign-gene introduction into specific cells are required for the functional analysis of specific cells. We report a new transfection method for intact plant cells that uses ArF excimer laser-induced shock waves to introduce foreign gene-coated gold particles into plant cells through the cell wall. Foreign gene (sGFP)-coated gold particles were randomly scattered on the surface of Torenia tissue samples; the laser was irradiated toward the gold particles using a hollow optical fiber and a quartz light-condensing tip to introduce the particles into the target cells. Treated plant tissue samples were then cultured for several weeks on the selection medium, and transformation was assessed by fluorescence microscopy and PCR. Irradiation-induced damage to the cells and coated DNA was insignificant, and the transformation efficiency was similar to that of biolistic transformation based on the particle number. Although this method requires a suitable laser setup and is time-consuming, it could be applied in transformation experiments on specific target cells. Moreover, this method can possibly be used to introduce not only DNA but also biologically active substances such as protein regulators or inhibitors, whose introduction into cells is difficult through the cell wall.
- 2007-06-01
著者
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Fukusaki Eiichiro
Department of Biotechnology, Graduate School of Engineering, Osaka University
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KOBAYASHI AKIO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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KAWABE Akira
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Kobayashi A
National Inst. Advanced Industrial Sci. And Technol. Ibaraki Jpn
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Kobayashi Akio
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.
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Kobayashi A
Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Inoue Fumihide
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Kobayashi A
Ochanomizu Univ. Tokyo Jpn
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Kobayashi A
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.
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Inoue Fumihide
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Fukusaki Eiichiro
Division Of Advanced Science And Biotechnology Graduate School Of Engineering Osaka University
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Fukusaki Ei-ichiro
大阪大学大学院
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Kajiyama Shin'ichiro
Department Of Biotechnology Graduate School Of Engineering Osaka University:school Of Biology-orient
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Kajiyama Shin'ichiro
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Shoji Takeshi
Department Of Biotechnology Faculty Of Engineering Osaka University
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Fukusaki E
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Fukusaki Eiichiro
Department Of Biotechnology Faculty Of Engineering Osaka University
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YOSHIKAWA Yoshinobu
Department of Biotechnology, Faculty of Engineering, Osaka University
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Kajiyama Shin-ichiro
Faculty Of Agriculture Okayama University
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Yoshikawa Yoshinobu
Department Of Biotechnology Faculty Of Engineering Osaka University
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Kobayashi Akio
Department Of Biotechnology Graduate School Of Engineering Osaka University
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KOBAYASHI Akio
Department of Biochemistry, Shinshu University School of Medicine
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KOBAYASHI Akio
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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