Transient RNAi Induction against Endogenous Genes in Arabidopsis Protoplasts Using in Vitro-Prepared Double-Stranded RNA
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概要
- 論文の詳細を見る
RNA interference is a powerful technique for suppressing gene functions in many eukaryotes including plants. Here we show that introduction of double-stranded RNA into Arabidopsis protoplasts leads to marked silencing of endogenous genes, as observed previously for transgenes [Biosci. Biotechnol. Biochem., 67, 2674–2677 (2003)]. This simple system should be useful for functional analysis of genes involved in fundamental cellular processes.
- 社団法人 日本農芸化学会の論文
- 2005-02-23
著者
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An Chung-il
Department Of Biotechnology Graduate School Of Engineering Osaka University
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FUKUSAKI EI-ICHIRO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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KOBAYASHI AKIO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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KAWABE Akira
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Kobayashi A
National Inst. Advanced Industrial Sci. And Technol. Ibaraki Jpn
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Kobayashi A
Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Kobayashi A
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.
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SAWADA Aki
Department of Biotechnology, Graduate School of Engineering, Osaka University
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KAWAGUCHI Yoshiaki
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Fukusaki Ei-ichiro
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Fukusaki Eiichiro
Division Of Advanced Science And Biotechnology Graduate School Of Engineering Osaka University
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Fukusaki Ei-ichiro
大阪大学大学院
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Sawada A
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Fukusaki E
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Sawada Aki
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kawaguchi Yoshiaki
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kobayashi Akio
Department Of Biotechnology Graduate School Of Engineering Osaka University
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KOBAYASHI Akio
Department of Biochemistry, Shinshu University School of Medicine
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KOBAYASHI Akio
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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