A Yeast Artificial Chromosome-Splitting Vector Designed for Precise Manipulation of Specific Plant Chromosome Region(Genetics, Molecular Biology, and Gene Engineering)
スポンサーリンク
概要
- 論文の詳細を見る
A yeast artificial chromosome (YAC) splitting vector, pKI01, was constructed for manipulating plant chromosome fragments cloned as YACs in order to transfer specific regions of the fragments into plant cells. Vector pKI01 consists of Km^r and ADE2 genes (selective markers for plant and yeast transformants, respectively), inverted telomeric repeats Tr and CEN4. To demonstrate the utility of pKI01, YAC CIC9e2 harboring a 590-kb fragment from Arabidopsis thaliana chromosome 5 was split into specific fragments. A 1-kb target region positioned 100 kb from the right end of the 590 kb fragment was cloned into pKI01. The resultant plasmid, pKY03, was introduced into Saccharomyces cerevisiae harboring YAC CIC9e2. The Ade^+ transformants were found to contain two new YACs of 490 and 100 kb, and to lack the original 590 kb YAC, consistent with the expected splitting event. To release the desired middle region of YAC CIC9e2, two additional splitting vectors were constructed, pKY11 and pKY14. By conducting two rounds of splitting, i. e., the first round 100 kb from the right end of YAC CIC9e2 with pKY11 to generate 490 and 100 kb YACs and a second round 50 kb from the right end of the new 490 kb YAC to generate 440 and 50 kb YACs, the middle 50 kb region of a plant chromosome fragment harboring Km^r was successfully released as a split YAC. These results indicate that YAC splitting vectors as constructed in this study are useful for generating any desired plant chromosome fragment as a YAC for eventual re-introduction into plant cells.
- 公益社団法人日本生物工学会の論文
- 2005-01-25
著者
-
Harashima Satoshi
Department of Biotechnology, Graduate School of Engineering, Osaka University
-
KOBAYASHI AKIO
Department of Biotechnology, Graduate School of Engineering, Osaka University
-
KAWABE Akira
Department of Biotechnology, Graduate School of Engineering, Osaka University
-
Kobayashi A
National Inst. Advanced Industrial Sci. And Technol. Ibaraki Jpn
-
Kobayashi A
Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University
-
Kobayashi A
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ.
-
Fukui K
Grad. Sch. Of Eng. Osaka Univ.
-
Harashima S
Department Of Biotechnology Graduate School Of Engineering Osaka University
-
Harashima Satoshi
Department Of Biotechnology Faculty Of Engineering Osaka University
-
Fukui Kiichi
Department Of Biotechnology Faculty Of Engineering Graduate School Of Osaka University
-
Kim Yeonhee
Department Of Biotechnology Graduate School Of Engineering Osaka University
-
Kaneko Yoshinobu
Department Of Biotechnology Graduate School Of Engineering Osaka University
-
Harashima Satoshi
Dept. Biotech. Grad. Sch. Eng. Osaka Univ.
-
Kobayashi Akio
Department Of Biotechnology Graduate School Of Engineering Osaka University
-
KOBAYASHI Akio
Department of Biochemistry, Shinshu University School of Medicine
-
KOBAYASHI Akio
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
関連論文
- Deciphering cellular functions of protein phosphatases by comparison of gene expression profiles in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Size Exclusion Chromatography of Standard Polystyrenes with a Wide Range of Molecular Weight Up to 7.45 × 10^6 on Monolithic Silica Capillary Columns
- Production of Eucommia-rubber from Eucommia ulmoides Oliv. (Hardy Rubber Tree)
- Introduction of the Archaebacterial Geranylgeranyl Pyrophosphate Synthase Gene into Chlamydomonas reinhardtii Chloroplast
- Application of the Bio-Active Beads Method in Rice Transformation
- An Arabidopsis thaliana Gene on the Yeast Artificial Chromosome Can Be Transcribed in Tobacco Cells
- Biosynthetic Pathway for the C45 Polyprenol, Solanesol, in Tobacco
- Isolation and Characterization of a cDNA Encoding Phytochrome A in the Non-Photosynthetic Parasitic Plant, Orobanche minor Sm
- Transient Gene Expression in Guard Cell Chloroplasts of Tobacco Using ArF Excimer Laser Microablation(PLANT BIOTECHNOLOGY)
- 3P-1006 Characterization of a suppressor of Δrrn10 disruptant causing the defect in rRNA transcription in Saccharomyces cerevisiae
- 2Bp14 Molecular breeding of Saccharomyces cerevisiae strain with high amount of RNA
- A Novel Gene Delivery System in Plans with Calcium Alginate Micro-Beads
- A Novel Gene Delivery System in Plants with Calcium Alginate Micro-Beads(PLANT BIOTECHNOLOGY)
- Isolation and Characterization of Two Novel Nematicidal Depsipeptides from an Imperfect Fungus, Strain D1084
- Functional Analysis of Two Solanesyl Diphosphate Synthases from Arabidopsis thaliana
- Transient RNAi Induction against Endogenous Genes in Arabidopsis Protoplasts Using in Vitro-Prepared Double-Stranded RNA
- A Transient RNA Interference Assay System Using Arabidopsis Protoplasts(Biochemistry & Molecular Biology)
- 3B11-1 A Rapid Comparison of Large-scale GC-MS Data for Metabolomics.
- 3B11-3 Metabolic Profiling of Camellia sinensis (Tea) Using GC/MS
- Obtaining transgenic plants using the bio-active beads method
- Guidelines for the treatment of Crohn's disease in children
- A New Organic Superconductor, λ-(BEDT-TSF)_2GaCl_4
- Molecular and functional characterization of novel furofuran-class lignan glucosyltransferases from Forsythia
- Molecular and functional characterization of novel furofuran-class lignan glucosyltransferases from Forsythia
- Comprehensive metabolite profiling of phyA phyB phyC triple mutants to reveal their associated metabolic phenotype in rice leaves(PLANT BIOTECHNOLOGY)
- High Throughput and Exhaustive Analysis of Diverse Lipids by Using Supercritical Fluid Chromatography-Mass Spectrometry for Metabolomics(METHODS)
- Quantitative Analysis of Anionic Metabolites for Cathamnthus roseus by Capillary Electrophoresis Using Sulfonated Capillary Coupled with Electrospray lonization-Tandem Mass Spectrometry(METHODS)
- Single Cell-Based Analysis of Torenia Petal Pigments by a Combination of ArF Excimer Laser Micro Sampling and Nano-High Performance Liquid Chromatography (HPLC)-Mass Spectrometry(METHODS)
- Pressure-Assisted Capillary Electrophoresis Mass Spectrometry Using Combination of Polarity Reversion and Electroosmotic Flow for Metabolomics Anion Analysis(METHODS)
- Stable Isotope Dilution-Based Accurate Comparative Quantification of Nitrogen-Containing Metabolites in Arabidopsis thaliana T87 Cells Using in Vivo ^N-Isotope Enrichment
- An Isotope Effect on the Comparative Quantification of Flavonoids by Means of Methylation-Based Stable Isotope Dilution Coupled with Capillary Liquid Chromatography/Mass Spectrometry(Methods)
- Two-Dimensional Quantitative Analysis of Preferential Alignment of BAp c-axis for Isolated Human Trabecular Bone Using Microbeam X-ray Diffractometer with a Transmission Optical System
- Fatty Acid Desaturation in Methylotrophic Yeast Hansenula polymorpha Strain CBS 1976 and Unsaturated Fatty Acid Auxotrophic Mutants
- Novel plant transformation system by gene-coated gold particle introduction into specific cell using ArF excimer laser
- Mass Mating Method in Combination with G418- and Aureobasidin A-Resistance Markers for Efficient Selection of Hybrids from Homothallic Strains in Saccharomyces cerevisias
- Genes in PHT Plasmid Encoding the Initial Degradation Pathway of Phthalate in Pseudomonas putida
- 3P-1005 Physiological characterization of high-temperature tolerant Saccharomyces cerevisiae strain and identification of a responsible gene
- Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Creating a Saccharomyces cerevisiae Haploid Strain Having 21 Chromosomes(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Oxazolomycin Esters, Specific Inhibitors of Plant Transformation
- Use of the PDR4 Gene as a Dominant Selective Marker in Combination with Cerulenin for Prototrophic Strains in Sacchromyces cerevisiae
- Plant Metabolomics : Potential for Practical Operation
- A Novel Bioassay Method for Inhibitors of Plant Transformation
- Production of a New Type of Bioactive Phenolic Compound
- Bisanthraquinones, Inhibitors of Plant Transformation
- ST-1, a Novel Radical Scavenger From Fungus F-124
- Quantitation of formate by solid-phase microextraction and gas chromatography-mass spectrometry utilizing a [^C]formate internal standard
- Methylamine Treatment Changes the Allocation of Carbohydrate to Roots in Rice Plants(ENVIRONMENTAL BIOTECHNOLOGY)
- Formate Dehydrogenase Gene of Arabidopsis thaliana is Induced by Formaldehyde and Not by Formic Acid
- Formate Protects Photosynthetic Machinery from Photoinhibition
- Formate Dehydrogenase in Rice Plant : Growth Stimulation Effect of Formate in Rice Plant
- An Artificial Plastic Receptor That Discriminates Axial Asymmetry
- New Antimicrobial Compound from Scorodocarpus borneensis Becc.
- Purification and Properties of Human D-3-Hydroxyacyl-CoA Dehydratase: Medium-Chain Enoyl-CoA Hydratase Is D-3-Hydroxyacyl-CoA Dehydratase
- Two Mitochondrial 3-Hydroxyacyl-CoA Dehydrogenases in Bovine Liver^1
- A Method for Direct Selection of Mating-Competent Clones from Mating-Incompetent Industrial Strains of Saccharomyces cerevisiae
- A System for Temperature-Controlled Expression of a Foreign Gene with Dual Mode in Saccharomyces cerevisiae
- A High-Throughput and Solvent-free Method for Measurement of Natural Polyisoprene Content in Leaves by Fourier Transform Near Infrared Spectroscopy(METHODS)
- Metabolic Profiling of Angelica acutiloba Roots Utilizing Gas Chromatography-Time-of-Flight-Mass Spectrometry for Quality Assessment Based on Cultivation Area and Cultivar via Multivariate Pattern Recognition(BREWING AND FOOD TECHNOLOGY)
- Quantification of trans-1,4-Polyisoprene in Eucommia ulmoides by Fourier Transform Infrared Spectroscopy and Pyrolysis-Gas Chromatography/Mass Spectrometry(METHODS)
- 1J10-4 ^1H NMR BASED METABOLIC PROFILING IN THE QUALITY EVALUATION OF JAPANESE GREEN TEA
- Effect of simvastatin on steroid-induced osteonecrosis evidenced by the serum lipid level and hepatic cytochrome P4503A in a rabbit model
- Improvement and Application of a Promoter-Probe Vector Bearing the PHO5 Gene as the Indicator Marker in Saccharomyces cerevisiae
- Partial Restoration of Sporulation Defect in Sake Yeasts, Kyokai No. 7 and No. 9,by Increased Dosage of the IME1 Gene
- The Transcriptional Activators of the PHO Regulon, Pho4p and Pho2p, Interact Directly with Each Other and with Components of the Basal Transcription Machinery in Saccharomyces cerevisiae^1
- Aroma Compounds in the Leaves of Japanese Pepper (Zanthoxylum piperium DC) and Their Formation from Glycosides
- Identification and Antimicrobial Activity of the Volatile Flavor Constituents from Scorodocarpus borneensis Becc
- A β-Hydroxychalcone and Flavonoids from Alfalfa Callus Stimulated by a Fungal Naphthoquinone, PO-1(Organic Chemistry)
- Unique Spindle Poisons, Curvularin and Its Derivatives, Isolated from Penicillium Species(Organic Chemistry)
- Metabolic Engineering of Lignan Biosynthesis in Forsythia Cell Culture
- 1019 Cloning and characterization of Δ 9-fatty acid desaturase genes from Pichia, Yarrowia and Kluyveromyces
- Effects of Dietary Nucleotide Supplementation on the Plasma Lipoprotein, Fatty Acids and Uric Acid in Early Infancy
- Effect of Dietary Nucleotide Supplementation on Development of the Faecal Flora in Newborn Infants
- Reaction of Copper Oxide and β-Diketone for In situ Cleaning of Metal Copper in a Copper Chemical Vapor Deposition Reactor
- Deposition Rate and Gap Filling Characteristics in Cu Chemical Vapor Deposition with Trimethylvinylsilyl Hexafluoro-acetylacetonate Copper (I)
- PHT, a Transmissible Plasmid Responsible for Phthalate Utilization in Pseudomonas putida
- A Simple Method for Detection of Enzyme Activities Involved in the Initial Step of Phthalate Degradation in Microorganisms
- Genetic Characterization of rbt Mutants That Enhance Basal Transcription from Core Promoters in Saccharomyces cerevisiae^1
- Effects of light on production of endogenous and exogenous lignans by Forsythia koreana wildtype and transgenic cells
- The construction of transgenic Forsythia plants: comparative study of three Forsythia species
- Control of Secretory Production of Human Lysozyme from Saccharomyces cerevisiar by Incubation Temperature and Phosphate Concentration
- Identification and Functional Characterization of Yeast ζ-COP
- Saccharomyces cerevisiae protein phosphatase Ppz1 and protein kinases Sat4 and Ha15 are involved in the control of subcellular localization of Gln3 by likely regulating its phosphorylation state(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Application of the PHO5-Gene-Fusion Technology to Molecular Genetics and Biotechnology in Yeast
- A Method for Fusing Chromosomes in Saccharomyces cerevisiae
- One-Step Splitting of a Chromosome in Haploid Cells of Saccharomyces cerevisiae and Its Effect on the Cell Proliferation
- Cloning and Characterization of Mevalonate Pathway Genes in a Natural Rubber Producing Plant, Hevea brasiliensis
- High-Throughput and Highly Sensitive Analysis Method for Polyisoprene in Plants by Pyrolysis-Gas Chromatography/Mass Spectrometry
- Cloning and Characterization of the 2-C-Methyl-D-erythritol 4-Phosphate (MEP) Pathway Genes of a Natural-Rubber Producing Plant, Hevea brasiliensis
- A Facile Transformation of Arabidopsis thaliana Using Ceramic Supported Propagation System
- Construction of a Saccharomyces cerevisiae strain with a high level of RNA(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- 29. Experimental Studies on the Phosphorous Metabolism of the Optic Nerve.
- A Yeast Artificial Chromosome-Splitting Vector Designed for Precise Manipulation of Specific Plant Chromosome Region(Genetics, Molecular Biology, and Gene Engineering)
- Repeated Chromosome Splitting Targeted to δ Sequences in Saccharomyces cerevisiae
- Lactic-acid stress causes vacuolar fragmentation and impairs intracellular amino-acid homeostasis in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Large-scale genome reorganization in Saccharomyces cerevisiae through combinatorial loss of mini-chromosomes(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Increased transcription of NOP15, involved in ribosome biogenesis in Saccharomyces cerevisiae, enhances the production yield of RNA as a source of nucleotide seasoning(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Genetic interactions of ribosome maturation factors Yvh1 and Mrt4 influence mRNA decay, glycogen accumulation, and the expression of early meiotic genes in Saccharomyces cerevisiae
- Disruption of multiple genes whose deletion causes lactic-acid resistance improves lactic-acid resistance and productivity in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)