Fatty Acid Desaturation in Methylotrophic Yeast Hansenula polymorpha Strain CBS 1976 and Unsaturated Fatty Acid Auxotrophic Mutants

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概要

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• Analysis of fatty acid composition in wild-type cells of Hansenula polymorpha strain CBS 1976 revealed the presence of 18 : 1(Δ9), 18 : 2(Δ9, 12)and18:3(Δ9, 12, 15)unsaturated fatty acids(UFAs), indicating that the α-linolenic desaturation pathway operates in this yeast. H. polymorpha cells also showed ability for uptake and incorporation of exogenous UFAs. By ethyl methanesulfonate mutagenesis, nine unsaturated fatty acid auxotrophic mutants of H. polymorpha were isolated. These mutants exhibited the growth arrest phenotype on nutrient medium and on nutrient medium supplemented with saturated fatty acids, but grew on media supplemented with various UFAs. Genetic analysis revealed that single recessive nuclear mutation conferred Ufa auxotrophy on these mutants. Fatty acid analysis by gas chromatography showed the accumulation of 18 : 0 but a decrease in the amount of 18 : 1 and 18 : 2 in mutant cells compared with the wild-type cells. Integrated physiological and genetical data suggested that mutations in all mutants occurred in one gene and probably led to defects in Δ9-desaturation pathway.

• 1998-05-25

著者

• Kaneko Yoshinobu

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Harashima Satoshi

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• ANAMNART Sarintip

  Department of Biotechnology, Osaka University

• Harashima S

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Harashima Satoshi

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Tolstorukov Ilya

  International Center Of Biotechnology Osaka University:state Research Center Of Russian Federation F

• Kaneko Y

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Kaneko Yoshinobu

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Harashima Satoshi

  Dept. Biotech. Grad. Sch. Eng. Osaka Univ.

• Anamnart Sarintip

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Anamnart Sarintip

  Department Of Biotechnology Faculty Of Engineering Osaka Universjty

• KANEKO Yoshinobu

  Department of Biotechnoiogy, Graduate School of Engineering, Osaka University

• HARASHIMA Satoshi

  Department of Biotechnoiogy, Graduate School of Engineering, Osaka University

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