A Structural and Phylogenetic Study of the HO Gene from Saccharomyces bayanus var. uvarum
スポンサーリンク
概要
- 論文の詳細を見る
A novel HO gene (Uv-HO) was cloned from the Saccharomyces bayanus var. uvarum (abbreviated as S. uvarum in this study) type strain. The coding region of Uv-HO showed relatively high homology (95%) to that of the Sb-HO gene (S. bayanus var. bayanus HO), but not to the HO genes of other Saccharomyces sensu stricto species. However, the 5′ and 3′ non-coding region of Uv-HO showed less similarity (79% and 76% respectively) even to those of the most homologous gene Sb-HO. Motifs of the mating-type control and the cell-cycle control were conserved in the 5′ non-coding region of Uv-HO, but numbers and positions of motifs were different from those of Sb-HO. CHEF-Southern analysis showed that all tested strains of S. bayanus species, including S. uvarum, carried the HO gene on the 1,100-kb chromosome. By HO-typing PCR using mixed primers, which provided a rapid and convenient tool for yeast identification, either the Uv-HO gene or the Sb-HO gene was detected in strains of S. bayanus species, but two strains were found to have both types of HO gene in each genome. These results suggest that S. uvarum has a unique sequence, but might share the same chromosome constitution within S. bayanus species, and that S. bayanus is a heterogeneous species, of which some strains might be natural hybrid.
- 社団法人 日本農芸化学会の論文
著者
-
Kaneko Yoshinobu
Department of Biotechnology, Graduate School of Engineering, Osaka University
-
TAMAI YUKIO
Central Laboratories for Key Technology, Kirin Brewery Co.Ltd.
-
KANAI Keiko
Central Laboratories for Frontier Technology, Kirin Holdings Co., Ltd.
関連論文
- Deciphering cellular functions of protein phosphatases by comparison of gene expression profiles in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Development of a Host-Vector System for Lactobacillus plantarum L137 Isolated from a Traditional Fermented Food Produced in the Philippines
- 3P-1006 Characterization of a suppressor of Δrrn10 disruptant causing the defect in rRNA transcription in Saccharomyces cerevisiae
- 2Bp14 Molecular breeding of Saccharomyces cerevisiae strain with high amount of RNA
- Accumulation of Compatible Solutes, Ectoine and Hydroxyectoine, in a Moderate Halophile, Halomonas elengata KS3 Isolated from Dry Salty Land in Thailand
- Phylogenetic Position of Mesorhizobium huakuii subsp.rengei, a Symbiont of Astragalus sinicus cv.Japan
- 1056 Intraspesific variation of Mesorhizobium strains isolated from Astragalus sinicus cv. Japan
- Characterization of Starch-Hydrolyzing Lactic Acid Bacteria Isolated from a Fermented Fish and Rice Food, "Burong Isda", and Its Amylolytic Enzyme
- Characterization of the ATF1 and Lg-ATF1 Genes Encoding Alcohol Acetyltransferases in the Bottom Fermenting Yeast Saccharomyces pastorianus
- A Structural and Phylogenetic Study of the HO Gene from Saccharomyces bayanus var. uvarum
- Pyruvate Decarboxylase Encoded by the PDC1 Gene Contributes, at Least Partially, to the Decarboxylation of α-Ketoisocaproate for Isoamyl Alcohol Formation in Saccharomyces cerevisiae
- Fatty Acid Desaturation in Methylotrophic Yeast Hansenula polymorpha Strain CBS 1976 and Unsaturated Fatty Acid Auxotrophic Mutants
- 3P-1005 Physiological characterization of high-temperature tolerant Saccharomyces cerevisiae strain and identification of a responsible gene
- Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Creating a Saccharomyces cerevisiae Haploid Strain Having 21 Chromosomes(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- 1102 Cloning and functional analysis of Δ^9-fatty acid desaturase gene of Hansenula polymorpha
- A Novel Hemiacetal Dehydrogenase Activity Involved in Ethyl Acetate Synthesis in Candida utilis
- Hemiacetal Dehydrogenation Activity of Alcohol Dehydrogenases in Saccharomyces cerevisiae
- A Simple PCR Method for Distinguishing Saccharomyces cerevisiae from Its Sibling species by Amplification of the RPL2 Region
- Genetic Characterization of rbt Mutants That Enhance Basal Transcription from Core Promoters in Saccharomyces cerevisiae^1
- Saccharomyces cerevisiae protein phosphatase Ppz1 and protein kinases Sat4 and Ha15 are involved in the control of subcellular localization of Gln3 by likely regulating its phosphorylation state(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Application of the PHO5-Gene-Fusion Technology to Molecular Genetics and Biotechnology in Yeast
- A Structural and Phylogenetic Study of the HO Gene from Saccharomyces bayanus var. uvarum
- Construction of a Saccharomyces cerevisiae strain with a high level of RNA(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- 2Hp11 Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol
- 1Fp06 Supraoptimal temperatures tolerance of Saccharomyces cerevisiae can be achieved by over-expression of RSP5 gene encoding ubiquitin ligase
- Lactic-acid stress causes vacuolar fragmentation and impairs intracellular amino-acid homeostasis in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Large-scale genome reorganization in Saccharomyces cerevisiae through combinatorial loss of mini-chromosomes(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Increased transcription of NOP15, involved in ribosome biogenesis in Saccharomyces cerevisiae, enhances the production yield of RNA as a source of nucleotide seasoning(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Genetic interactions of ribosome maturation factors Yvh1 and Mrt4 influence mRNA decay, glycogen accumulation, and the expression of early meiotic genes in Saccharomyces cerevisiae