Accumulation of Compatible Solutes, Ectoine and Hydroxyectoine, in a Moderate Halophile, Halomonas elengata KS3 Isolated from Dry Salty Land in Thailand
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概要
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A moderately halophilic and halotolerant eubacterium, strain KS3, was isolated from a salty soil in Thailand and identified as Halomonas elongata based on the results of conventional taxonomic and DNA-DNA reassociation studies. H.elongata KS3 grows in the presence of 0.3 to 21% NaCl and at temperatures of 5 to 45℃. The optimum concentration of NaCl and temperature for growth were 3 to 5% and 37℃, respectively. Under hyperosmotic stress in a glucose-mineral medium, the accumulation of several low-molecular weight nitrogenous organic compounds was observed. These compounds were identified as 1, 4, 5, 6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid(ectoine), 1, 4, 5, 6-tetrahydro-2-methyl-5-hydroxy-4-pyrimidinecarboxylic acid(hydroxyectoine), γ-N-acetyl-α, γ-diaminobutyric acid(ADABA), and L-alanine. Ectoine production was induced immediately by the addition of NaCl and the amount accumulated increased with an increasing external concentration of NaCl and reached approximately 120 μg・mg^<-1> of dry cells at a concentration of 2.56 M NaCl. Hydroxyectoine was detected with a lag in time after the addition of NaCl at a concentration greater than 1.71 M, and the amount accumulated increased and reached 45μg・mg^<-1> of dry cells at a concentration of 2.56 M NaCl. Thus, hyperosmotic stress induced by salt appears to cause the accumulation of ectoine and hydroxyectoine as compatible in H.elongata KS3.
- 社団法人日本生物工学会の論文
- 1998-04-25
著者
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Kaneko Yoshinobu
Department of Biotechnology, Graduate School of Engineering, Osaka University
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MUROOKA YOSHIKATSU
Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech
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Murooka Yoshikatsu
Department Of Biotechnology Graduate School Of Engineering Osaka University:hiroshima Institute Of T
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Murooka Y
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Ono Hisayo
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Ono Hisayo
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Ono Hisayo
Department Of Fermentation Technology Osaka University
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SHINMYO Atsuhiko
Department of Biosciences, Nara Institute of Science and Technology
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Ono H
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Ono Hisayo
Department Of Biotechnology Faculty Of Engineering Osaka University
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Ono H
Manufacturing Technology Laboratories:fujisawa Pharmaceutical Co. Ltd.
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OKUDA MITSUYOSHI
Department of Biotechnology, Graduate School of Engineering, Osaka University
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TONGPIM SAOWANIT
Department of Biotechnology, Graduate School of Engineering, Osaka University
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IMAI KO
Institute of Fermentation
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SAKUDA SHOUHEI
Department of Biotechnology, Graduate School of Engineering, Osaka University
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TAKANO MITSUO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Imai Ko
Institute For Fermentation
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Kaneko Y
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kaneko Yoshinobu
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Takano M
Institute Of Genetic Ecology Tohoku Universtiy
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Sakuda Shouhei
Department Of Biotechnology Graduate School Of Engineering Osaka University:(present Address)departm
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Murooka Yoshikatsu
Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.
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Tongpim Saowanit
Department Of Biotechnology Graduate School Of Engineering Osaka University:(present Address)departm
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Okuda Mitsuyoshi
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Takano Mitsuo
Department of Biotechnology, Faculty of Engineering, Osaka University
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SAKUDA Shouhei
Department of Agricultural Chemistry, The University of Tokyo
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KANEKO Yoshinobu
Department of Biotechnoiogy, Graduate School of Engineering, Osaka University
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