Cloning and Nucleotide Sequencing of New Glutaryl 7-ACA and Cephalosporin C Acylase Genes from Pseudomonas Strains
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概要
- 論文の詳細を見る
We cloned a gene for the glutaryl 7-ACA acylase from Pseudomonas sp. A14 and genes for the cephalosporin C acylases from Pseudomonas diminuta N176 and V22 in Escherichia coli and determined their nucleotide sequences. Single open reading frames were recognized, which were composed of 2457 base pairs for the A14 gene and 2322 base pairs for the N176 and V22 genes. We also purified and characterized these enzymes from recombinant E. coli strains. Each enzyme was shown to be composed of two non-identical subunits. Determination of N-terminal amino acid sequences revealed that both subunits were encoded within an open reading frame, suggesting that both subunits of these enzymes are produces from a common precursor peptide. Comparison of amino acid sequences among these enzymes and other known glutaryl 7-ACA acylase and penicillin G acylase showed that there was 10 to 25% homology distributed heterogeneously along the sequences.
- 社団法人日本生物工学会の論文
- 1991-10-25
著者
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Ono Hisayo
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Ono Hisayo
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Ono Hisayo
Department Of Fermentation Technology Osaka University
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Aramori Ichiro
Pharmacological Research Laboratories Fujisawa Pharmaceutical Co. Ltd.
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Aramori Ichiro
Exploratory Research Laboratories Fujisawa Pharmaceutical Co. Ltd.
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Ishii Y
Pharmacological Research Laboratories And Exploratory Research Laboratories
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KOJO Hitoshi
Advanced Technology Platform Research Laboratory Fujisawa Pharmaceutical Co., Ltd.
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FUKAGAWA Masao
Advanced Technology Platform Research Laboratory Fujisawa Pharmaceutical Co., Ltd.
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FUKAGAWA MASAO
Exploratory Research Laboratories
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TSUMURA MANA
Exploratory Research Laboratories
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IWAMI MORITA
Exploratory Research Laboratories
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ONO HIROKI
Manufacturing Technology Laboratories
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ISHITANI YOSUKE
Manufacturing Technology Laboratories
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KOJO HITOSHI
Exploratory Reseach Laboratories
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KOHSAKA MASANOBU
Research and Development Group
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UEDA YOSHIO
Research and Development Group
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IMANAKA HIROSHI
Research and Development Group
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ISOGAI TAKAO
Exploratory Research Laboratories
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Ishii Y
Fermentation Development Laboratories Fujisawa Pharmaceutical Co. Ltd.
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Kojo H
Advanced Technology Platform Research Laboratory Fujisawa Pharmaceutical Co. Ltd.
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Fukagawa Masao
Advanced Technology Platform Research Laboratory Fujisawa Pharmaceutical Co. Ltd.
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Ono Hisayo
Department Of Biotechnology Faculty Of Engineering Osaka University
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Ono H
Manufacturing Technology Laboratories:fujisawa Pharmaceutical Co. Ltd.
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ISOGAI TAKAO
Fujisawa Pharmaceutical Co. Ltd.,
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KOHSAKA MASANOBU
Pharmacological Research Laboratories and Exploratory Research Laboratories
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Ueda Yoshio
New Drug Research Laboratories Fujisawa Pharmaceutical Co. Ltd.
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Iwami Morita
Exploratory Research Laboratories:fujisawa Pharmaceutical Co. Ltd.
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Isogai Takao
Fujisawa Pharmaceutical Co. Ltd.
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Tsumura Masanori
Exploratory Research Laboratories:fujisawa Pharmaceutical Co. Ltd.
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