Promotion of Metal Accumulation in Nodule of Astragalus sinicus by the Expression of the Iron-regulated Transporter Gene in Mesorhizobium huakuii subsp. rengei B3(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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概要

• 論文の詳細を見る

• Toxic metal contamination in agricultural fields is an important worldwide problem. In previous studies, we developed a bioremediation system based on the symbiosis between Astragalus sinicus and the recombinant rhizobium, Mesorhizobium huakuii subsp. rengei B3 developed by overexpressing a synthetic tetrameric metallothionein gene(MTL4) and cDNA encoding the phytochelatin synthase from Arabidopsis thaliana(AtPCS). To promote the transport of metals into the nodules of the rhizobium and the accumulation of metals, the iron-regulated transporter 1 gene from A. thaliana(AtIRT1) was introduced into recombinant strain B3 containing MTL4 or AtPCS in its chromosome. The fused AtIRT1-alkaline phosphatase was expressed in the free-living recombinant rhizobium and the nodule of A. sinicus. The recombinant strain B3 carrying AtIRT1 showed a higher Cd sensitivity and a higher amount of Cd accumulated in free-living culture than the wild-type strain B3. When the recombinant strain B3 established symbiosis with A. sinicus, the introduction of AtIRT1 in the recombinant strain B3 advantaged the accumulation of Cu and As in the nodules of A sinicus, compared with that of Cd and Zn.

• 社団法人日本生物工学会の論文
• 2008-06-25

著者

• YAMASHITA MITSUO

  Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech

• MUROOKA YOSHIKATSU

  Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech

• Yamashita Mitsuo

  Department Of Biotechnology Graduate School Of Engineering Osaka University:department Of Applied Ch

• Yamashita M

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Murooka Yoshikatsu

  Department Of Biotechnology Graduate School Of Engineering Osaka University:hiroshima Institute Of T

• Murooka Y

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Ono Hisayo

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Ike Akiko

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Sriprang Rutchadaporn

  BIOTEC Central Research Unit

• Ike Akiko

  Department Of Biotechnology Graduate School Of Engineering Osaka University:(present Office)faculty

• Ono Hisayo

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Ono H

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Ono Hisayo

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Murooka Yoshikatsu

  Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.

• Matsumoto Yukimasa

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Yamashita Mitsuo

  Department Of Agricultural Chemistry Faculty Of Bioresources Mie University Department Of Biotechnol

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