In Vivo Cloning of Arylsulfatase-Tyramine Oxidase Genes in rec Strains of Klebsiella aerogenes

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概要

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• A general, genetic technique for in vivo cloning of bacterial genes is presented. Wepreviously introduced the Muphage into various genera of bacteria including Klebsiella aerogenes with RP4: : Mu.Using these strains carrying RP4: : Mu cts and thermo-inducible Muprophage in the chromosome, we cloned in vivo the arylsulfatase (ats) and tyramine oxidase (tyn) genes by partial thermo-induction. The donor strains carrying the recombinant plasmids were conjugated with K. aerogenes rec strains, which were isolated as UV-sensitive mutants. The resultant recombinant plasmids, pATl and pAT2, were purified and used for the transformation of mutant strains deficient in the ats and tyn genes. The ats-tyn genes seemedto be transposed into the RP4::Mu cts plasmid together with other chromosomal DNA fragments. This in vivo cloning method is applicable to a wide variety of gram-negative bacteria.

• 社団法人 日本農芸化学会の論文

著者

• MUROOKA YOSHIKATSU

  Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech

• Murooka Yoshikatsu

  Department Of Biotechnology Graduate School Of Engineering Osaka University:hiroshima Institute Of T

• Murooka Y

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• OKA Masahide

  Institute of Scientific and Industrial Research, Osaka University

• YAMASHITA Mitsuo

  Institute of Scientific and Industrial Research, Osaka University

• SUGIYAMA Masanori

  Department of Fermentation Technology, Faculty of Engineering, Hiroshima University

• HARADA Tokuya

  Institute of Scientific and Industrial Research, Osaka University

• Harada Tokuya

  Institute Of Scientific And Industrial Research Osaka University

• Sugiyama Masanori

  Department Of Fermentation Technology Faculty Of Engineering Hiroshima University

• Murooka Yoshikatsu

  Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.

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