Phylogeny of Bacterial Symbionts of the Laguminous Tree Acacia mangium

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概要

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• Bacterial strains isolated from root nodules on Acacia mangium at various locations in Indonesia were characterized by restriction fragment length polymorphism (RFLP) analysis of PCR-amplified 16 rDNA and by an intergenic repetitive extragenic palindromic (REP) sequence. The RFLP-16S rDNA analysis used four restriction endonucleases classified into seven Sau3AI, eight TaqI, nine HinfI, and nine MspI groups. Analysis of the amplified REP-PCR products showed intra-specific variations in the rhizobial genomic DNA. From the data obtained, 10 of the 15 strains isolated from A. mangium were classified as Bradyrhizobium. These isolates clustered with B. elkanii, and were predominant in the A. mangium nodulation systems. Partial 16S rDNA sequence analysis of some representative strains demonstrated that the RFLP clusters were stable and phylogenetically relevant.

• 社団法人日本生物工学会の論文
• 1997-12-25

著者

• Fujie Makoto

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• Yamada Takashi

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• SUKIMAN HARMASTINI

  R & D Center for Biotechnology, LIPI

• YAMASHITA MITSUO

  Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech

• MUROOKA YOSHIKATSU

  Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech

• Nuswantara Sukama

  Department Of Agricultural Chemistry Faculty Of Bioresources Mie University Department Of Biotechnol

• Murooka Yoshikatsu

  Department Of Biotechnology Graduate School Of Engineering Osaka University:hiroshima Institute Of T

• Murooka Y

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Fujie M

  Graduate School Of Advanced Sciences Of Matter Hiroshima University

• Fujie M

  Hiroshima Univ. Higashi‐hiroshima Jpn

• Fujie Makoto

  Department Of Molecular Biotechnology Graduate School Of Engineering Hiroshima University

• Fujie Makoto

  Department Of Fermentation Technology Faculty Of Engineering Hiroshima University

• Yamada Takashi

  Department Of Chemistry Faculty Of Science And Engineering Konan University

• NUSWANTARA SUKMA

  Department of Fermentation Technology, Faculty of Engineering, Hiroshima University

• Sukiman Harmastini

  R & D Centre For Biotechnology Indonesian Institute Of Sciences (lipi)

• Yamada T

  Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive

• Murooka Yoshikatsu

  Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.

• Yamashita Mitsuo

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Yamashita Mitsuo

  Department Of Agricultural Chemistry Faculty Of Bioresources Mie University Department Of Biotechnol

• Yamada Takashi

  Department Of Animal Science Graduate School Of Agricultural Science Kobe University

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