Monitoring of Phytopathogenic Ralstonia solanacearum Cells Using Green Fluorescent Protein-Expressing Plasmid Derived from Bacteriophage φRSS1(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)

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概要

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• A green fluorescent protein (GFP)-expressing plasmid was constructed from a filamentous bacteriophage φRSS1 that infects the phytopathogen Ralstonia solanacearum. This plasmid designated as pRSS12 (4.7kbp in size) consists of an approximately 2248bp region of the φRSS1 RF DNA, including ORF1-ORF3 and the intergenic region (IG), and a Km cassette in addition to the GFP gene. It was easily introduced by electroporation and stably maintained even without selective pressure in strains of R. solanacearum of different races and biovars. Strong green fluorescence emitted from pRSS12-transformed bacterial cells was easily monitored in tomato tissues (stem, petiole, and root) after infection as well as from soil samples. These results suggest that pRSS12 can serve as an easy-to-use GFP-tagging tool for any given strain of R. solanacearum in cytological as well as field studies.

• 社団法人日本生物工学会の論文
• 2007-12-25

著者

• Fujie Makoto

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• Kawasaki Takeru

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• Yamada Takashi

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• Usami Shoji

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• Satsuma Hideki

  Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive

• Fujie M

  Graduate School Of Advanced Sciences Of Matter Hiroshima University

• Fujie M

  Hiroshima Univ. Higashi‐hiroshima Jpn

• Fujie Makoto

  Department Of Molecular Biotechnology Graduate School Of Engineering Hiroshima University

• Usami S

  Graduate School Of Advanced Sciences Of Matter Hiroshima University

• KAWASAKI Takeru

  Dept. Mol. Biotech., Grad. Sch. Adv. Sci. Mat., Hiroshima Univ.

• Kawabata Toyoki

  Energia Economic And Technical Research Institute

• Kawasaki Takeru

  Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive

• Kawasaki Takeru

  Department Of Molecular Biotechnology Graduate School Of Advanced Matter Hiroshima University

• Fujie Makoto

  Department Of Fermentation Technology Faculty Of Engineering Hiroshima University

• Yamada Takashi

  Department Of Chemistry Faculty Of Science And Engineering Konan University

• Usami Shoji

  Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University

• Yamada T

  Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive

• Yamada T

  National Institute Of Agrobiological Sciences Ministry Of Agriculture Forestry And Fisheries

• Yamada Takashi

  Department Of Animal Science Graduate School Of Agricultural Science Kobe University

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