Resolvase-like serine recombinase mediates integration/excision in the bacteriophage φRSM(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
- 論文の詳細を見る
φRSM1, a filamentous phage infecting Ralstonia solanacearum, encodes an open reading frame (ORF14) that exhibits significant homology to members of the resolvase/invertase subfamily of site-specific serine recombinases. Similar prophages are found in the genomes of various strains of R. solanacearum, R. pickettii, and Burkholderia pseudomallei. We have characterized the integrative and excisive recombination reactions mediated by the φRSM1 integrase using in vivo assays. An E. coli plasmid containing the RSM1 orf14 and attP sequences (pT-orf14-attP) was shown to integrate into the attB sequence of R. solanacearum cells. Intermolecular recombination between pT-orf14-attP and pS-attB (a R. solanacearum plasmid containing attB) also occurred in R. solanacearum cells. The products of attP/attB recombination, i.e., attL and attR, were exactly identical to the sequences found in the prophage φRSM in R. solanacearum strains. Intramolecular recombination between the attL and attR sequences (excisive recombination) on the pRecomb plasmid was also shown to occur in an orf14-dependent manner. This is the first evidence that a small serine recombinase from the resolvase/invertase group may function in integrative and excisive recombination for filamentous phages.
著者
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Fujie Makoto
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Kawasaki Takeru
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Yamada Takashi
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Fujie M
Graduate School Of Advanced Sciences Of Matter Hiroshima University
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Fujie M
Hiroshima Univ. Higashi‐hiroshima Jpn
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Fujie Makoto
Department Of Molecular Biotechnology Graduate School Of Engineering Hiroshima University
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KAWASAKI Takeru
Dept. Mol. Biotech., Grad. Sch. Adv. Sci. Mat., Hiroshima Univ.
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Kawabata Toyoki
Energia Economic And Technical Research Institute
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Kawasaki Takeru
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Kawasaki Takeru
Department Of Molecular Biotechnology Graduate School Of Advanced Matter Hiroshima University
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Fujie Makoto
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Yamada Takashi
Department Of Chemistry Faculty Of Science And Engineering Konan University
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Yamada T
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Askora Ahmed
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Askora Ahmed
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Yamada T
National Institute Of Agrobiological Sciences Ministry Of Agriculture Forestry And Fisheries
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Yamada Takashi
Department Of Animal Science Graduate School Of Agricultural Science Kobe University
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