ZMVHA-B1, the Gene for Subunit B of Vacuolar H^+-ATPase from the Eelgrass Zostera marina L. Is Able to Replace vma2 in a Yeast Null Mutant(PLANT BIOTECHNOLOGY)

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概要

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• A vacuolar H^+-ATPase (VHA) gene (ZMVHA-B1) was isolated from an eelgrass (Zostera marina) leaf cDNA library and was characterized to be approximately 1.4 kbp in length and to encode the B subunit protein of VHA comprising 488 amino acids. ZMVHA-B1 was highly expressed in all organs of eelgrass; the expression level was highest in the leaves. On transformation of a yeast vma2 null mutant with ZMVHA-B1, yeast cells became able to grow at pH 7.5, accompanied by the vesicular accumulation of LysoSensor green DND-189. Thus, ZMVHA-B1 expressed in yeast cells produced a functional B subunit that was efficiently incorporated into the VHA complex and eventually restored vacuolar morphology and activity. This success expedites the application of heterologous expression in yeast mutant cells to the screening of eelgrass genes involved in salt-resistance mechanisms, which are to be utilized in improving important crops.

• 2006-11-25

著者

• Fujie Makoto

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• Yamada Takashi

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• Alemzadeh Abbas

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• Usami Shoji

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• Yoshizaki Tsukasa

  Energia, Economic and Technical Research Institute

• Oyama Keiji

  Energia, Economic and Technical Research Institute

• Kawabata Toyoki

  Energia, Economic and Technical Research Institute

• Oyama Keiji

  Energia Economic And Technical Research Institute

• Fujie M

  Graduate School Of Advanced Sciences Of Matter Hiroshima University

• Fujie M

  Hiroshima Univ. Higashi‐hiroshima Jpn

• Fujie Makoto

  Department Of Molecular Biotechnology Graduate School Of Engineering Hiroshima University

• Usami S

  Graduate School Of Advanced Sciences Of Matter Hiroshima University

• Alemzadeh Abbas

  Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive

• KAWASAKI Takeru

  Dept. Mol. Biotech., Grad. Sch. Adv. Sci. Mat., Hiroshima Univ.

• Yoshizaki Isukasa

  Energia Economic And Technical Research Institute

• Kawabata Toyoki

  Energia Economic And Technical Research Institute

• Kawasaki Takeru

  Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive

• Fujie Makoto

  Department Of Fermentation Technology Faculty Of Engineering Hiroshima University

• Yamada Takashi

  Department Of Chemistry Faculty Of Science And Engineering Konan University

• Usami Shoji

  Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University

• Yamada T

  Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive

• Yoshizaki Tsukasa

  Energia Economic And Technical Research Institute

• Yamada T

  National Institute Of Agrobiological Sciences Ministry Of Agriculture Forestry And Fisheries

• Yamada Takashi

  Department Of Animal Science Graduate School Of Agricultural Science Kobe University

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