Two Catalytic Domains of Chlorella Virus CVK2 Chitinase
スポンサーリンク
概要
- 論文の詳細を見る
VChti-1 chitinase encoded by the Chlorella virus CVK2 contained two catalytic domains belonging to family 18 glycosyl hydrolases. The first catalytic domain on a C-terminal-truncated derivative of vChti-1 generated exclusively chitobiose from chitotetraose, chitohexaose, and colloidal high-molecular mass chitin in the enzyme reaction, a typical characteristic of an exochitinase. In contrast, N-acetylglucosamine was produced from chitobiose as well as from chitooligosaccharides by the second catalytic domain on an N-terminal-truncated derivative of vChti-1. Therefore, the second domain possessed N-acetylglucosaminidase activity as well as endochitinase activity. The presence of two catalytic domains with different enzymatic properties in the viral enzyme seems to be necessary for hydrolyzing natural substrates in a cooperative fashion.
- 社団法人日本生物工学会の論文
- 2000-03-25
著者
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Fujie Makoto
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Yamada Takashi
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Usami Shoji
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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SAKAI Kazuo
Yaizu Suisan Kagaku Industry Co.
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Fujie M
Graduate School Of Advanced Sciences Of Matter Hiroshima University
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Fujie M
Hiroshima Univ. Higashi‐hiroshima Jpn
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Fujie Makoto
Department Of Molecular Biotechnology Graduate School Of Engineering Hiroshima University
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Usami S
Graduate School Of Advanced Sciences Of Matter Hiroshima University
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HIRAMATSU SHINGO
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Hiramatsu Shingo
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Fujie Makoto
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Yamada Takashi
Department Of Chemistry Faculty Of Science And Engineering Konan University
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Usami Shoji
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda University
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Yamada T
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Yamada T
National Institute Of Agrobiological Sciences Ministry Of Agriculture Forestry And Fisheries
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Sakai Kazuo
Yaizu Suisankagaku Industry Co.ltd.
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Yamada Takashi
Department Of Animal Science Graduate School Of Agricultural Science Kobe University
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