Mapping of cDNA Clones on Contigof Chlorella Chromosome I
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概要
- 論文の詳細を見る
Complementary DNA (cDNA) clones specific to the smallest chromosome (chromosome I) of Chlorella vulgaris C-169 were selected from cDNA libraries with probes of chromosome I DNA fragments amplified by depenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). A total of 15 clones was obtained, which included gene homologs for α-tubulin, inosine-5-monophosphate dehydrogenase, β-1, 4-mannase, a TTG-binding protein, a heat shock protein, thioredoxin/protein disulfide isomerase, franscription factor NF-E2, an oxidoreductase, and UDP-n-acetylglucosamine enolpyruvyltransferase. These clones were definitely localized at specific sites on the chromosome I physical map constructed on the basis of overlapping cosmid clones (the contig). They were predominantly distributed within the left two-thirds of the chromosome. This contrasts with the distribution of repetitive elements such as short interspersed elements (SINEs), which are rather abundant in the right two-thirds of chromosome I. The comparative simplicity of the gene arrangement of Chlorella chromosome I suggest that it may be able to serve as a prototypic system for deciphering the complexity of huge plant chromosomes.
- 社団法人日本生物工学会の論文
- 2000-10-25
著者
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Fujie Makoto
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Yamada Takashi
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Fujie Makoto
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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OHTA Yutaka
Department of Electrical and Electronic Engineering, Faculty of Engineering, Tokyo Institute of Tech
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Fujie Makoto
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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MAKI SINYA
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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NOUTOSHI YOSHITERU
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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USAMI SHOHJI
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Yamada Takashi
Department Of Chemistry Faculty Of Science And Engineering Konan University
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Maki Sinya
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Usami Shohji
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Ohta Yutaka
Department Of Electrical And Electronic Engineering Faculty Of Engineering Tokyo Institute Of Techno
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Ohta Yutaka
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Noutoshi Yoshiteru
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Yamada Takashi
Department Of Animal Science Graduate School Of Agricultural Science Kobe University
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Yamada Takashi
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima University
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